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mmu_ciIws1_605
Basic information of circular RNA
PanCircBase ID:
mmu_ciIws1_605
mm39_Chr. Position ID:
chr18|32223593|32224198|+
Gene name:
Iws1
Transcript:
ENSMUST00000025243.5
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
11
Exon/intron offsets:
0
Genomic length:
605
Exon/intron sizes in nt:
605
Flanking exon/introns:
chr18:32223593-32224198
Splice length:
605
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATGAGGGAACAGGTATGAATGCACAAGTGTAGCTTAATCTGATGGCATCTAGGGAAGACCTTTCTGGGACAGCTGAATACAATATGCTATCCCAGGGCTGCAGTGCTAGAGATGGTAGTGTTCTAGACATTTGCAGAAAACAACTTTCTCAGCCCAGTTCCACAACTGTAAGAGGAAAAAAATGTTATTGGTTTAATTTCTCTGTATTCTGGATATATTTTGGAAGGCAGGGCTATCATTTATAAGTATAGTTTGGGTAGGTCACCCAGAATGAAACCTCCTGCACAGTACAGTTACAGTGAACAATACGTGAGAACAGTGACAAGTTCTCAGGCCAGCAAGATGGTTCAGAGCAGAAAGGCACTTGCTGTGCAAGTCTGGCAACCTGAGTGATCCGCATGACCCAAGTAAAGGTGGAAGGAAAACTAACTCCAGTTATCCTCTGGCCACATGCATACTCTCACACACATACTTTTAATTAATTGTTTTTGTATGGTTTAGTATAAAGTATTAGTTATAATATAAATGTGATATTTTGACGTGCTTTCCCCTAGTTCTAAGAATTTGAGATTTCTGGTATCTCACTGTTTGCTCTTTTTCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGACGTGCTTTCCCCTAGTTC
60
TCAGCTGTCCCAGAAAGGTC
59.31
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGACGTGCTTTCCCCTAGTTC
60
CAGCTGTCCCAGAAAGGTCT
59.31
144
PCR template sequence:
TAAAGTATTAGTTATAATATAAATGTGATATTTTGACGTGCTTTCCCCTAGTTCTAAGAATTTGAGATTTCTGGTATCTCACTGTTTGCTCTTTTTCCAGGTATGAGGGAACAGGTATGAATGCACAAGTGTAGCTTAATCTGATGGCATCTAGGGAAGACCTTTCTGGGACAGCTGAATACAATATGCTATCCCAGGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-216a-5p(1), mmu-miR-382-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-539-5p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCTCTTTTTCCAGGTATGAGGGAACA
siRNA sets
TCATACCTGGAAAAAGAGC, CTCATACCTGGAAAAAGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.77004685
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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