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mmu_ciIft74_1170
Basic information of circular RNA
PanCircBase ID:
mmu_ciIft74_1170
mm39_Chr. Position ID:
chr4|94580017|94581187|+
Gene name:
Ift74
Transcript:
ENSMUST00000030311.11
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
19
Exon/intron offsets:
0
Genomic length:
1170
Exon/intron sizes in nt:
1170
Flanking exon/introns:
chr4:94580017-94581187
Splice length:
1170
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATCCTTTAAACTGATGTGGCTCGGCCTTATTGAATGCTTAATGTGTACAGAACTCTGAGTTAATTATATGGGGGTCACAAAGACTAGCACCAGTTTCTGTTCTGTAACGCTGTTTACTCAGACTATTAGAAAACTGATTTTGAGAATTACCCAAATGGAGTATAAAGTCACCATATAACATAGTGCTTAAGGGCATACATTATAGAGTTGGATGATCAAATTTTGAATGGTGATTCTGTCACTGGTAAGCTACAGTTCTTAGAAAGTTCTTTTGATCGGTTTAAGCCTTAATTTTATAATGTCTAGAACAGTTTGTATTCATAGAATTTCTGAAATAGTTTAGATATAGGTCTTAGTTGTTTACAATGCTACAACTTGGCTGTCTATAGAGCAAAGGGTGCTAACCAGTCAGGGTCATAAGAAATTTGACCTCTGAGTTCAATTATGTTACACATGATTCAACTGCATCTTATTCCTACCAAGTGAGGGCCTGCATTGTAGAGTTACAGCATCTGTCTTTGTTTCCTGCCTGTTCCCTGTGACTTATTCCAGGACATCTGTCCTCAGAGCAAGATGGTTATCACAGTTGTCTTTTAGGGTGTTGCTGAACTTTATCTCAGAGGCAGGACACACCTCAGCTAACTTCTGGATAGGATGCCGTAGGAGTTCTATCCAAGTGTTAGAACAACTCAGCTTAAAAGGCTGACTGGTTATTCACTGGATGTTTAAAAGTCCAGCATTCTAGCGTTGTGCAACATAAATGACATGATTCTGACCTAGTGAAGTCTTGAGACTTGCCTGAAATTCCTTGTGACTGTATGGAGACTAGAGGTTTTCTGAATGTTAGTTGTTCTGAGATTAGAATTAGATGGAGCAGCTGATAGTACAAAGTCAGGAGCTGGAGGAGAGGAGGCTGTGATCAGGCTGTAAAGTGAATGAATAAATAATGAGGAAAAAAATCAAAAGTGATTTTTTTGAAACAAGTTTTACTTGGCCTTTTTGTGTCGATTTATGCTTTTCTACAATAAGGTTAAATGTAAAATTTATTCTAGTACTTTTTCTGTACTGTCTACATTCACTGTGACAAATACCCAAGTCTGCTTCAGTTCCTTTGAGTCACAGGCTGCTGTTTTTACTGACATTGTTCCTCCTTAACTCTTGTAATTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGTCACAGGCTGCTGTTT
59.82
GCTAGTCTTTGTGACCCCCA
59.31
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGTCACAGGCTGCTGTTT
59.82
TGCTAGTCTTTGTGACCCCC
59.31
147
PCR template sequence:
TCTACATTCACTGTGACAAATACCCAAGTCTGCTTCAGTTCCTTTGAGTCACAGGCTGCTGTTTTTACTGACATTGTTCCTCCTTAACTCTTGTAATTAGGTATCCTTTAAACTGATGTGGCTCGGCCTTATTGAATGCTTAATGTGTACAGAACTCTGAGTTAATTATATGGGGGTCACAAAGACTAGCACCAGTTTCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-30e-5p(1), mmu-miR-101a-3p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-216a-5p(1), mmu-miR-128-3p(1), mmu-miR-30b-5p(1), mmu-miR-136-5p(1), mmu-miR-382-5p(1), mmu-miR-511-3p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCTTGTAATTAGGTATCCTTTAAAC
siRNA sets
TTAAAGGATACCTAATTAC, TAAAGGATACCTAATTACA, AAAGGATACCTAATTACAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.859543317
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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