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mmu_ciHuwe1_900
Basic information of circular RNA
PanCircBase ID:
mmu_ciHuwe1_900
mm39_Chr. Position ID:
chrX|150677014|150677914|+
Gene name:
Huwe1
Transcript:
ENSMUST00000026292.15
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
42
Exon/intron offsets:
0
Genomic length:
900
Exon/intron sizes in nt:
900
Flanking exon/introns:
chrX:150677014-150677916
Splice length:
900
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTCAGTTTCATTTGCTACTATTCTTTCAGAAAATTCTCTTTAGGCAGATATTGAGACATTGGTTGAGCATTTGACTTAGTAAATTCTGTATGTAATTCTCAGTAATTTCTCTTGGCCTCTTAATACAACTCCCAAGGATTCACTCTAGTCTTTACCTACAAAGTGTTCATTTTGATGTGACAATAGAAGTTAAATTAACTGCAGTTGGTTTCTGTATAGTGCGATCATTGAAAAACAATTGTTTGTTTGGGTGACAGAATCCAATGAAAAGTTTACAGTGCTACCTGTATTCTTACCAAAAAGTTAATGCCTGTATTTCATTGAGGAAGTAATTACATTTATATCAAATTATGGAAATATTTTACTGAACAGCTAGCCTAAACCCATGGTAAACACGAATTGAAAGGAAAAATTGAAGTATCTCAGCAGATGAGAACGGTCACTTCTACTTGGATTGGATTCTGGATCCATTTCTAGGAGACTTGTAGGATATATGAAGTTTCTCAGAAGTTCATACAGACATTTAAAAAATTGTGAGATTGTTTATTGAAATATAAAAACATTTCACCGGGCAGTGGTGGCGCATGCCTTTAATCCCAGCACTTGGGGAGGCAGAGTCAGGTGAATTTCTGAGTTCAAGGCCAGCCTGGCCTACAGAGTTAGTTTCAGGACAGCCAGAGCTACACAGAGAAACCCTGTCTTGAAAAACTTAAAAAAACAAAACAAAATTTGTTTATAGTTTATAAAAGGTAGATTTCTTTACTATAAGACTTGAACTTAATTTTTAAAGAATTATTTCTCCTGCTTTTTATGTACTACACAATCCTTGTTCTGGGTACTTTCTTGTTGATAGTGAATACCAGTGTTCTATTGTCTGACTTTACTGTTTAATTTTGGCCT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACACAATCCTTGTTCTGGGT
56.95
GTCAAATGCTCAACCAATGTCTCA
60.02
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACACAATCCTTGTTCTGGGT
56.95
TCAAATGCTCAACCAATGTCTCA
58.86
157
PCR template sequence:
CCTGCTTTTTATGTACTACACAATCCTTGTTCTGGGTACTTTCTTGTTGATAGTGAATACCAGTGTTCTATTGTCTGACTTTACTGTTTAATTTTGGCCTGTCAGTTTCATTTGCTACTATTCTTTCAGAAAATTCTCTTTAGGCAGATATTGAGACATTGGTTGAGCATTTGACTTAGTAAATTCTGTATGTAATTCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-539-5p(2), mmu-miR-200a-3p(1), mmu-miR-203-3p(1), mmu-miR-495-3p(1), mmu-miR-362-5p(1), mmu-miR-324-3p(1), mmu-miR-384-3p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
TTAATTTTGGCCTGTCAGTTTCATTT
siRNA sets
AAATGAAACTGACAGGCCA, AATGAAACTGACAGGCCAA, AAACTGACAGGCCAAAATT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.94331642
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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