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mmu_ciHspa5_293
Basic information of circular RNA
PanCircBase ID:
mmu_ciHspa5_293
mm39_Chr. Position ID:
chr2|34665245|34665538|+
Gene name:
Hspa5
Transcript:
ENSMUST00000028222.13
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
8
Exon/intron offsets:
0
Genomic length:
293
Exon/intron sizes in nt:
293
Flanking exon/introns:
chr2:34665245-34665562
Splice length:
293
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAATTAAACGTTCACAAATATTGTATGTTTTGAAAGGATGTGATCTGGGTTTCACAATATTATTAAACAATATTATTAAATAAGTTAAACTATTATCCTCCCAGGGAAAGAAGTATGTTTTGTAACTCAAATGAAGAGGTTCTTAGTTGAACCAGTGTTGAATTTTTTTCATGTTAACTAGCTTTTGTAATACAAGGTCAGAGGGGCTTACAATGCTTTGAAGAGTTGAAATTAAGAGCAGTAGCACCCAGTGAGTTAATGTAGGGATCCCAGTGCCCTGGCTTTGTCAATA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAAGGTCAGAGGGGCTTACA
59.02
GTGAAACCCAGATCACATCCT
57.64
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTCAGAGGGGCTTACAATGCT
60.07
GTGAAACCCAGATCACATCCT
57.64
152
PCR template sequence:
CAAGGTCAGAGGGGCTTACAATGCTTTGAAGAGTTGAAATTAAGAGCAGTAGCACCCAGTGAGTTAATGTAGGGATCCCAGTGCCCTGGCTTTGTCAATAGTAATTAAACGTTCACAAATATTGTATGTTTTGAAAGGATGTGATCTGGGTTTCACAATATTATTAAACAATATTATTAAATAAGTTAAACTATTATCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-221-3p(1), mmu-miR-335-5p(1), mmu-miR-222-3p(1), mmu-miR-3087-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCTTTGTCAATAGTAATTAAACGTT
siRNA sets
AACGTTTAATTACTATTGA,AATTACTATTGACAAAGCC,TAATTACTATTGACAAAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.904264908
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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