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mmu_ciHipk2_2532

Basic information of circular RNA
PanCircBase ID:

mmu_ciHipk2_2532

mm39_Chr. Position ID:

chr6|38693156|38695688|-

Gene name:

Hipk2

Transcript:

ENSMUST00000161779.8

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

11

Exon/intron offsets:

0

Genomic length:

2532

Exon/intron sizes in nt:

2532

Flanking exon/introns:

chr6:38693156-38695688

Splice length:

2532

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAGGTGATGAGAGCCAGCAAGAAACACCAGAAGATTGAAATGCTAAACACCACTTGGAAGTAGGGCTTGCCAGTGCACTGCCCTGGCCTGGTGCAGGGAGCAGCTCTTCCTACAAGAGCTGGGAACATCTGGCAGCACATGGAACACTCTGGAATTTGCTAAAAGCACTCTCTCTGGGCTCTGTGCCTCTTGACTCTTCCTAGTGCCTGTCCCTCTGGCACTCTAAGGTGGAGGCCTAGAGTCATGGTGCACCTAATGTCTTGAGTGCTTCAGTCATTTTACCAAAGAAAAAGTAGTTAAAGCACAGTAAGGAAGATTTTATTTGAGACCACAGGGATGGATACAAAGATGCTGCAGTGGAGGAGAAAGAGTGGGCTCAGCTCTACACATAGCATGGGCATCGAGAGTTTGTACCCAGGGAGTAGTGTCTAGGTCACATAGGGAAAATAGCAGATAGGAGATGGCAGAAACTGGAGGATTGTAGCTAAACAACCCTAACAAGATTCTCTGAAGGTGATTAGCCATCACCTAAGGGAGAGTGGGGGAGGAGGAATCTGGTCAGACATTAGGTCTAGTATTCTAGATATCAAGAGATGGGAAGAGTGTCCTCTAGCTAAAATGAATTAGCAAGGTTCTTAGCTAAAACTAGATTTTATAAAGAAGTGCACAGTTGAGCCTATGAAAAGGTTCAAAGTCCTGCCTAAATTTGGCCACACAGTCTCTGTCGTCTTTTCTAGAAACAGCAAACCTTTGAAGTTTCCATTTGATTACAAATTATCAAGATATATTTTTAAAAATCACTAAAAAATGTACTTTTTTGAAATTTCAGGTCAGAAACATAACTCATTAACTTAAAAATATGTTCTATAGTTATGCTTATCTTACTTCGACCTCTTTACTTAAAAGGTGCTAGACTCTCTAAGGACATAGCAGAGCCAAAGGTGTATTCTCTCTTGCCTTTGGTCTTTACTGCATGGGCACTAACCAGGATCAGCAAAGGGTTTGATGGGAAGAAAAGAAGGTAATTGTTCCCTAGAGTTACCAGAACTTAATATAGAATTTCAGGTCAATCAGTGAAGTTCTCTTTGATGGGCTAGAAAGATGGCTCACTGGTTAAGAGCACTGACTACTCTTCTGGAGGATCCAGGTTTAATTCCCAAACCCTATGGTAGCTCACAACCATCTGTAACTCCAGTCCCAGGGGACCTGATACCGTCTTCCTAACCTCCTAGGGCACCAGGGTGCACATAATGCAGACAAAACACTCATACACATGAAATGATGAATAAATCTAAAACTAATAATTAATTTTAAAAGAGTTAAACTATGTATGCTACAATACTCAGATTTGGCTTATCCATTTTTTTTTTAAATTTTTCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGAAACTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCTGCCTGTCTCTGCCTCCCAAGTGCTGTGATTAAAGGCATGCACCAGTGTACCTTCTTCCTCTTACATTTGTCCTAATTTTTTATTGCAATACAAATTAATTGATAAGCCTGCCATGAGGCTTAGGTTAAGATCCAAAAAGTTTTGTTTTTCTTTGTTTCCCATTGCACATCCCCAAATCTGAATAGCTATGGCCTTACCAGAAGAGAAAGAAGGGAACGCTGAGCCAAGGGGCAGTGGAGATCACTCCATCTGTAAATGCTTTCTACACAAGCACAAAGACCTACGTTTAGTCACTAGAACCTGCATAAAAAAGCTGAGCATGGCAGTACACACCTGTGCCGAGTGCTGAGGTTAGAGACAAGCAGACCCTGGGGAACTTGGGCAATCAGCTAAATCAGTGGGCTCCAGTAAGGGAACTGTCTTCTCTCAAAAAACAAGATGAACAGCTCTTAAGGAACATACACCTGAGGTTGACCTCTGGCATGCGCGTGTACACACATACACACACACACACACACACACACACACACACACACACACACACACCTGTTCATTAGTCTAGAAACTCTTACAAGTGTCTTAAGGTAGCAGTGTGGCTCCATGGCTCCAGGCTAGACTATCTAATTCAATCCCAGCACAGAATTGGGGGCTAGTAATTAAATAATAAACCAACCATCAGGAAGAAAATTCTCACCACTCCTAAAACAAATACCATTGAGGGAGGGCTAGATGAAGCCAAAGTCTGGGCTTTAGTCCTGAAGATTGTCTTATGCAGCTGTGTTGGATCTGACAGCTAAAATCCTGATCAGGACAGCTGTCTCTAAAAGACCAGCTCTCAAAAACAGGTATATGGACCCAAACCAGAGCCCAGAGAAGGCCCAGCAGAGGTCCTAACCTCAGTCCTGAATGAAGGCCTCTAGAAGCACTGTATTTTCTGTCACAAGGGAGGTCAACTGTCATGGGCTTCAGATTTTCTCCTGTGGAGATGTGGTAACGTTGGTGTTTTATTTTAAAGTTAGATGGAGATGTAGGGCCAGACCCTCCTTGTAAATCGTGTTGTTTTGTCATTACAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

CCTGTGGAGATGTGGTAACGT

59.72

TGGCAAGCCCTACTTCCAAG

59.96

170

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

CCTGTGGAGATGTGGTAACGT

59.72

CTGGCAAGCCCTACTTCCAA

59.96

171

PCR template sequence:

TCTCCTGTGGAGATGTGGTAACGTTGGTGTTTTATTTTAAAGTTAGATGGAGATGTAGGGCCAGACCCTCCTTGTAAATCGTGTTGTTTTGTCATTACAGGTAGGTGATGAGAGCCAGCAAGAAACACCAGAAGATTGAAATGCTAAACACCACTTGGAAGTAGGGCTTGCCAGTGCACTGCCCTGGCCTGGTGCAGGGA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

27

Interacting miRNAs (#binding sites)

mmu-miR-1187(4), mmu-miR-9-5p(2), mmu-miR-574-5p(2), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-205-5p(1), mmu-miR-152-3p(1), mmu-miR-148a-3p(1), mmu-miR-18a-5p(1), mmu-miR-328-3p(1), mmu-miR-709(1), mmu-miR-326-3p(1), mmu-miR-185-5p(1), mmu-miR-339-5p(1), mmu-miR-10b-5p(1), mmu-miR-149-5p(1), mmu-miR-434-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-218-5p(1), mmu-miR-324-3p(1), mmu-miR-34b-5p(1), mmu-miR-297a-5p(1), mmu-miR-466a-5p(1), mmu-miR-466p-5p(1), mmu-miR-532-5p(1), mmu-miR-669d-5p(1), mmu-miR-490-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TTTGTCATTACAGGTAGGTGATGAGA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.775726923

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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