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mmu_ciHeatr5b_1485
Basic information of circular RNA
PanCircBase ID:
mmu_ciHeatr5b_1485
mm39_Chr. Position ID:
chr17|79132175|79133660|-
Gene name:
Heatr5b
Transcript:
ENSMUST00000097281.4
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
7
Exon/intron offsets:
0
Genomic length:
1485
Exon/intron sizes in nt:
1485
Flanking exon/introns:
chr17:79132174-79133660
Splice length:
1485
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGTAATGCACATGCGTGAGTGGGCTCTGTCTGGATCATTGCATGAGAGACTGTCGTAAGAGCAAATCGGAACATTTTAACAGCCTGCTTTCTTTGAAACATTTTTAACCAATGAATGGATTAAATCCAGATTGCAAATCTCATCGCATACTTATAAGTAATGGTAGATTCTCCTTTTTTCTTATGTCTCTTTTGGGGGGGGGGAATACTGTGAACTGATATTAGGGCCTTATACATACTAGGCAAGCAAATACCCTACCACTGAGCTATATCCTGGTCTGAGAGTTAAATTCTTGATGTATAAATGTCTATTTTTACTATATGTACATAAATATCTAAAAAATATTCTCAAATTTTATTTCACAACAAATAAGTTATAAAGTAGGCTTTGCCTAGCATGGTAGTGTAGGCCACAGGCTGAGACAGGGGGATCTTTAGTTCAAAGCCAGCCTAAGTCCCATCATGGAGACTTTACTCAAAAAAACATAAACATGAAATAATAAAAGTGAAACTAGATACAATATAATCCTTTAAAAAATAATAAAGTAAGACAGTTTTGTTTAGAACTAAATTTAGACATTACTAGTTTAGACATTTAGACTAACTTAGATTCCATAGACCTTGTGATTTGATTGATTTATGCCTCTTATTGGATTTGTCAGTGACTAATCTGTAATGAATTATAAAATCAAATATGACTTGGATTTATAATTTCAAAATAATAACTAATATTTACAGATCTGATACCATCTAGTAAGTTTGCATGGCTCATGGTCTTAGTCAGTGTCACCATGAAGGTATCTTGTCCCTGACACAAAATGTACAGTGGAAGCCATCTGCTTATCTCAGGAACTCTGGCAAGAATGCTGCAGCTCTTAGTATCTATTTGCAATGGTAACTTCCATTCCATAAGTTGTTACTTTGCTGATCCTGACTGAGTTTAGCTCTGCTGTCCACCTTAGCCCGGTCTCTTTACTTCTTCATGAGTGGCTTGTCCTCGAGACTCTTACGGTGTTTTGACTTTGATTTTTAGATAGAGTCTTTCACTCTGTTGCCCAGGCTGCCCTTGGACTTGAGGTGATTGCCCTGAAGGCTGAGATCGCAGGCACGCAATACAATGCCTGGCTCTTTTCAATAATTTTGTATTCAAGCCAGGCATAATAGCACGTGCCTTTAATCCTAGTAGAGGCAGGCAGTTCTTTGTGAGTTCAAGCCTGGCCTACATAGTGAGTTCCAGGCCAGCAAAGGTTCCATGAGACTCTGATTGCAAATGAACAAAAACAAACGAAAAGAATTGTCTCATTCAAACATTCTACCAGTAATTCCTTTGGTAGAAGTCGTAAAAAGATGTGAAGTTCATCTCTGCTGTTTAATCTCAGGTCTAACTTGTTGTGTTTGCTTCCATCGCTGTTTGGCTGTCGTTTTCTGTGCTGCTTTCCGTCCACCCAGAGGTACCTAAAGCTGTGTCTTAATGTGTTTCCTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTGTCGTTTTCTGTGCTG
60.04
GCAGGCTGTTAAAATGTTCCGA
59.77
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTGTCGTTTTCTGTGCTG
60.04
AGCAGGCTGTTAAAATGTTCCG
59.77
161
PCR template sequence:
AACTTGTTGTGTTTGCTTCCATCGCTGTTTGGCTGTCGTTTTCTGTGCTGCTTTCCGTCCACCCAGAGGTACCTAAAGCTGTGTCTTAATGTGTTTCCTAGTTTGTAATGCACATGCGTGAGTGGGCTCTGTCTGGATCATTGCATGAGAGACTGTCGTAAGAGCAAATCGGAACATTTTAACAGCCTGCTTTCTTTGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-124-3p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-103-3p(1), mmu-miR-15b-5p(1), mmu-miR-184-3p(1), mmu-miR-107-3p(1), mmu-miR-27a-5p(1), mmu-miR-3087-3p(1), mmu-miR-452-3p(1), mmu-miR-3470b(1), mmu-miR-344-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AATGTGTTTCCTAGTTTGTAATGCAC
siRNA sets
ATTACAAACTAGGAAACAC, TTACAAACTAGGAAACACA, CATTACAAACTAGGAAACA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.845650319
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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