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mmu_ciGolga3_666
Basic information of circular RNA
PanCircBase ID:
mmu_ciGolga3_666
mm39_Chr. Position ID:
chr5|110355638|110356304|+
Gene name:
Golga3
Transcript:
ENSMUST00000112512.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
15
Exon/intron offsets:
0
Genomic length:
666
Exon/intron sizes in nt:
666
Flanking exon/introns:
chr5:110355638-110356305
Splice length:
666
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAGGTAAACACATTTCTCCCCGTCAAAGTCCTTAGGACTGTTGGTTTTCTTTAGTTGTGTTTTTTGTTTTTCAAGACAGGATTTTTCTGTGTAGCAGCCCTGGCTGTCCTGGAACTTGCTTTGTAGACCAAGCTGGCCTTGAACTCACACTGGGCTGAAGCAGGACGATCAGAATTTCAAGGTTAATCTTGGCTGTATGGCAAATTCTAAGCCAGCATGAGCTAAATGAGACCTATATCAAACAAAAGAGTAGAGCCTTCTGAGTTGGGTTTAGTAATGCAATCCCAACACTTGTGAGGCTGAGGGGAGAAGTTCACTACTTCAGAGCCATCCTGCGCTGTTTAGTAAGGAGACTGTGTTTCAGAAACTAAAACAACTGGGCTAGTTGAATGGCTTAGTGGGTACAGGCCCCTTCCACCATGCCTGATGACTTGAGTTTGATTCCCAGTCACATTATAGGAGTCAGGAGAGACCTGACACCCAAAAGTTGTCCTTTGAACTGTGCATGCACACAAGAACACCTACACACATGCACATACACATAACAGTAAATGAATAACGTGGAATAAAAATCTTAAAAATCTAGAATGTGATCAGGTATGTGTCCATGAGAGTTGAAGGAGGAAAAGGCCAGTGACTAAATATGTCTCCCACTTTGCCTTTCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAGGAAAAGGCCAGTGACT
59.6
TGCTACACAGAAAAATCCTGTCT
58.03
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAGGAAAAGGCCAGTGAC
59.6
TGCTACACAGAAAAATCCTGTCT
58.03
144
PCR template sequence:
ATAAAAATCTTAAAAATCTAGAATGTGATCAGGTATGTGTCCATGAGAGTTGAAGGAGGAAAAGGCCAGTGACTAAATATGTCTCCCACTTTGCCTTTCAGTAGGTAAACACATTTCTCCCCGTCAAAGTCCTTAGGACTGTTGGTTTTCTTTAGTTGTGTTTTTTGTTTTTCAAGACAGGATTTTTCTGTGTAGCAGCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-23a-3p(1), mmu-miR-297a-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-207(1), mmu-miR-669d-5p(1), mmu-miR-452-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTTTGCCTTTCAGTAGGTAAACACA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.704792899
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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