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mmu_ciGnai3_382
Basic information of circular RNA
PanCircBase ID:
mmu_ciGnai3_382
mm39_Chr. Position ID:
chr3|108019406|108019788|-
Gene name:
Gnai3
Transcript:
ENSMUST00000000001.5
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
6
Exon/intron offsets:
0
Genomic length:
382
Exon/intron sizes in nt:
382
Flanking exon/introns:
chr3:108019404-108019788
Splice length:
382
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATGTTGACGATTGGGGTAGAAAGTCCATCTGATACAGCTAGGAAGTGAGCGTGACTCAAGGAAGACGGGTGCCACTGCTGGCTCACGGAACCAAAACTTAATGCCCTGTATTTTCTTGTCAGTGGGGCTAGAGTTCCCAAATCCTTGTTCCATTGCACATTTGCTCTTGGTAGCCACATTGTGTGCACAGGATTGCATCTTGTCATCTTCTTAACCCCTTACCCCAAGAATTATGCAAATCAGGACTGACTAGGCTTCAAGTCACATTAGGACCTACTCTATTTCCATGAATTCAGTTTGTTGTTTTAAAACTTCTTGGTATGTGAACTAATCTGCTTTTTATTCTTGCTGCTAATCTGTCATAACCATGTTTGTTTTTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGCTTTTTATTCTTGCTGCT
56.87
AGTTTTGGTTCCGTGAGCCA
60.11
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGCTTTTTATTCTTGCTGCT
56.87
GTTTTGGTTCCGTGAGCCAG
59.69
148
PCR template sequence:
ATTTCCATGAATTCAGTTTGTTGTTTTAAAACTTCTTGGTATGTGAACTAATCTGCTTTTTATTCTTGCTGCTAATCTGTCATAACCATGTTTGTTTTTCGTATGTTGACGATTGGGGTAGAAAGTCCATCTGATACAGCTAGGAAGTGAGCGTGACTCAAGGAAGACGGGTGCCACTGCTGGCTCACGGAACCAAAACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-335-5p(1), mmu-miR-18a-5p(1), mmu-miR-495-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGTTTGTTTTTCGTATGTTGACGAT
siRNA sets
AACATACGAAAAACAAACA, CAACATACGAAAAACAAAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.842798529
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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