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mmu_ciGm37885_2286
Basic information of circular RNA
PanCircBase ID:
mmu_ciGm37885_2286
mm39_Chr. Position ID:
chr1|182311855|182314141|+
Gene name:
Gm37885
Transcript:
ENSMUST00000192568.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
2286
Exon/intron sizes in nt:
2286
Flanking exon/introns:
chr1:182311855-182316125
Splice length:
2286
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTACAGCCTCTCTCCCCATCCTCCTCATCTTCGTCTTCTTCCTCCAGCTTAATTAGGTACTGAGGGTTCATCCAGAAGGTATCTGTAGAACAGGGTCAGAGGAACCCAGCTCTGAATAAATAAATGTCCAGGAGCTGAGAACTGGGGCGGGCCTGGCCTTGTGGCTGAGCTGGTCAGTCGAGGATGAGAAAAAAGGAGCCGGTGGGAGACGAGGAGGCACAGAAGAGGCCAAAGGCAACCAACCCTTTAGATGGAGTGGCTACTGACAGAGGTGTGCTAGGCCTGAAGCTATCATGAGGAGACTGCTTCCTCGGGTTCTCTCCTCTGGCACCAAGTTCTTATAGCTCAAGCTGGCCTCAAACTTGCTTATGTCTCTGAAGATGAGCTTGGATTTCTGATCTCCTGACTCTACCTCTGAGTGCAGGGGTTGCAAGTACTAGGAACTGAACGGGGGCACCAGGCATGTGAGGTAACCACTCCCATCAGAGTTACGTTCTTGGTCTCATTACAAAGTCTTCCTGAAGGCTTATCACCCTTGTCATAGCAAATGGAGGGCCACTGGCACAGTATAAATCTCAGGAGGACCTAAATCAGGAATTGTTCTTTTTTTTCTGCCCTTACGCCGCTGGCTTGCCTTGATGCTCCATCTTCCCACACTGCCTGTCTTTTGGCCCCCACCCCTTGGCAAATGCAAAGACCAGAAAGCATCTGTGTCTCTTGCTCACCATCACAGTGCCTCTACAACCCATACTTCCATCTGCAGGAAGACCCTGAATCTCACCCCCAACCCTTGGTCTGAGTGCCTCCCACCCCTCATGCAATGACCTCTGCGGTAGAATTAGAAGCACGGTCTCACAGTGTTGGCAGCCATGTTACAACTGCTTCTTTAAACTCAGTTGTGTTGGCACACACGTGTGTATGAAGCTCTAGGCTACCTCGGTGGCACTGAGTAAGTACTGTAGAGGCAGAGGGTCCCCAAGGCCAAGAGCATTCACTCTGAGGTCCTTGAGAGAGCATCTGCGGACCTCCCATCTAACGGGTACAGGGTGCTCCTGTGGGTGGCAGAGGGGCTTCTCAGTGGTAGCCTCTAGAACTGCCACTTGGTGACTGCGTGCTGGGACTGGAGGGACTGGCCAGCTAGTCTACTTGCTCTGTTGGTCTGGTATATCACCTCGAACCTCTGTCTCCTCATCTGCAACAAGAGTCAATATATGACCATGGCCTGCCTCCAGAGTAAGAGTGACTTCGTAGCCACATCGCCTGGGTTCAAACCCCAGAAAGCTCTGTGGCCTTGGACCAGCCACTTCCCTTGGCCCTGTCCCGTCATCCTCAAGTTGGAAAGGAGAATGGGTGACTTCCTCGAGAAGATTAATACATAGATGAAGCTCTTTGGGACAGTTCCCAGTACTCTATGCACACTCCATCCTACGACTGGCAGTGTCATCGAGATCAGACAGGGTCTAGGGGACAAAGAGGAGGAGAGGACTGAGCTGTGAGGACAACAGAAGAGATCAGCAAAGGGATCAAGAGAGCAGAGCAGAGGAGGAGCCCTCTCCTGACACGGGCTGGCCTCATCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTTCCTCCCTCCTTCCCTCCTCTCTCTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTCCCAGTCTTTTTGAGACAGCGATTCTCTGTGTAGCCCTGGCTGTCCTGGAACCCACTCTGTACAGCACAATGACCTCCACCTCCAAGATCCTCCTGTCTCTGCCTCCCAAGTGTTGGGATTAAAGGAGTACAGCTACCAACACCCAGCTCTAAACATTTTACAAGTGAACTTGACACACAAGAGAGTGGCAGACTGCCCTGAGAGGCTGGTGTGCCCCAGGATGCATTACATGAGGGCCTGGGATGGCTCGTTATGAACTAGGACATAGCACTGCCTCCATAGCCAGATGAAGGCCACAGCCACCAGGGGATGTGGGGACACCAGGGGACATCACAGCAAATGAATGCAGGACGTAGTAAGGCCGAGAAAACGGAAAAAAGATGTTGACACACAGGCTGGTGGTTAAGGAAGAGAATGAGAGCCTGGGTGAGTGGGTGCCAAGTCCCCTGGGCTTCCAGGCTGAGTGGCATATCTAGTCAGGTAGGAGGCCACGGGACCGGAGCCACACATGGTCATGGCCCTTACTTGGGTAATTCCTGCAGCCCCCTGCGGTGGAGC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TAGTCAGGTAGGAGGCCACG
60.4
CCTTCTGGATGAACCCTCAGT
59.09
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TAGTCAGGTAGGAGGCCACG
60.4
ACCTTCTGGATGAACCCTCAG
59.09
165
PCR template sequence:
GCTGAGTGGCATATCTAGTCAGGTAGGAGGCCACGGGACCGGAGCCACACATGGTCATGGCCCTTACTTGGGTAATTCCTGCAGCCCCCTGCGGTGGAGCGTACAGCCTCTCTCCCCATCCTCCTCATCTTCGTCTTCTTCCTCCAGCTTAATTAGGTACTGAGGGTTCATCCAGAAGGTATCTGTAGAACAGGGTCAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
37
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(2), mmu-miR-185-5p(2), mmu-miR-34b-5p(2), mmu-miR-296-3p(2), mmu-miR-140-5p(1), mmu-let-7a-5p(1), mmu-miR-7b-5p(1), mmu-miR-34a-5p(1), mmu-miR-101a-3p(1), mmu-miR-122-5p(1), mmu-let-7c-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-101b-3p(1), mmu-miR-7a-5p(1), mmu-miR-709(1), mmu-miR-203-3p(1), mmu-miR-324-5p(1), mmu-miR-10b-5p(1), mmu-miR-139-5p(1), mmu-miR-10a-5p(1), mmu-miR-181b-5p(1), mmu-let-7d-5p(1), mmu-miR-136-5p(1), mmu-miR-433-3p(1), mmu-miR-676-3p(1), mmu-miR-96-5p(1), mmu-miR-425-5p(1), mmu-miR-98-5p(1), mmu-miR-690(1), mmu-miR-384-3p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-669d-5p(1), mmu-miR-484(1), mmu-miR-452-3p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTGCGGTGGAGCGTACAGCCTCTCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIGM37885_2286_ORF_1
1.0524
-0.12118729
0.083589866
207
ORF sequence:
ATGAGAGCCTGGGTGAGTGGGTGCCAAGTCCCCTGGGCTTCCAGGCTGAGTGGCATATCTAGTCAGGTAGGAGGCCACGGGACCGGAGCCACACATGGTCATGGCCCTTACTTGGGTAATTCCTGCAGCCCCCTGCGGTGGAGCGTACAGCCTCTCTCCCCATCCTCCTCATCTTCGTCTTCTTCCTCCAGCTTAATTAGGTACTGA
Peptide sequence:
MRAWVSGCQVPWASRLSGISSQVGGHGTGATHGHGPYLGNSCSPLRWSVQPLSPSSSSSSSSSSLIRY
IRESfinder result
IRESfinder score
0.739989114
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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