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mmu_ciGm26749_286
Basic information of circular RNA
PanCircBase ID:
mmu_ciGm26749_286
mm39_Chr. Position ID:
chr17|74914755|74915041|-
Gene name:
Gm26749
Transcript:
ENSMUST00000181387.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
286
Exon/intron sizes in nt:
286
Flanking exon/introns:
chr17:74882575-74915041
Splice length:
286
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTACACAGAGTTACATGGCCAAAATACACAATGTATCATTAAAATTATATGCAGTTTAAAGAAAAAAAAAAAAGGTTGGGTTGTCACTCAGTGACAAGGCACTTGCCTCAATGACCAGTCTCACCACGGATATGTTCATATGAGCACTGCAATGCAGTGATACTACCACTGCTACACCAATTTTACAGTGAGGTATTGGAGCAATTCCCAATATTTTTCCCTCTTGAAGGAAGTTTTTTAGTGAAGCCCACAATTAAAAGACTTTGAATTTTAAAGTACTTTGAAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGAAGCCCACAATTAAAAGACT
57.55
TCACTGAGTGACAACCCAACC
60.13
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGAAGCCCACAATTAAAAGAC
56.16
TCACTGAGTGACAACCCAACC
60.13
140
PCR template sequence:
AGTGAGGTATTGGAGCAATTCCCAATATTTTTCCCTCTTGAAGGAAGTTTTTTAGTGAAGCCCACAATTAAAAGACTTTGAATTTTAAAGTACTTTGAATGTACACAGAGTTACATGGCCAAAATACACAATGTATCATTAAAATTATATGCAGTTTAAAGAAAAAAAAAAAAGGTTGGGTTGTCACTCAGTGACAAGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-33-5p(1), mmu-miR-411-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAGTACTTTGAATGTACACAGAGTTA
siRNA sets
TAACTCTGTGTACATTCAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIGM26749_286_ORF_1
0.382
-0.481878254
0.002948372
96
ORF sequence:
ATGTACACAGAGTTACATGGCCAAAATACACAATGTATCATTAAAATTATATGCAGTTTAAAGAAAAAAAAAAAAGGTTGGGTTGTCACTCAGTGA
Peptide sequence:
MYTELHGQNTQCIIKIICSLKKKKKGWVVTQ
IRESfinder result
IRESfinder score
0.786930524
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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