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mmu_ciGm26658_147
Basic information of circular RNA
PanCircBase ID:
mmu_ciGm26658_147
mm39_Chr. Position ID:
chr18|31576754|31576901|+
Gene name:
Gm26658
Transcript:
ENSMUST00000181067.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
147
Exon/intron sizes in nt:
147
Flanking exon/introns:
chr18:31576754-31579945
Splice length:
147
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGATTGTCATTTTGATGTACGGGTCAGAGGTCATGGATTGCTCGTCCATGGCTGGTAAGCCCTGGGCTTCCTTGATGTTCACCACAAATGCTTTCTTCTCGAAGTTGTACTCTAGAGACAAGAAGAGTGTCCCCAGCTTCTCTT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGATGTTCACCACAAATGCT
55.84
TGGACGAGCAATCCATGACC
60.11
123
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGATGTTCACCACAAATGCT
55.84
GGACGAGCAATCCATGACCT
59.82
122
PCR template sequence:
TTGATGTTCACCACAAATGCTTTCTTCTCGAAGTTGTACTCTAGAGACAAGAAGAGTGTCCCCAGCTTCTCTTGTAAGATTGTCATTTTGATGTACGGGTCAGAGGTCATGGATTGCTCGTCCATGGCTGGTAAGCCCTGGGCTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-27a-5p(1), mmu-miR-99a-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCCAGCTTCTCTTGTAAGATTGTCAT
siRNA sets
ATCTTACAAGAGAAGCTGG, CAATCTTACAAGAGAAGCT, AATCTTACAAGAGAAGCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIGM26658_147_ORF_1
1.2004
0.167989524
0.416023752
276
ORF sequence:
ATGTACGGGTCAGAGGTCATGGATTGCTCGTCCATGGCTGGTAAGCCCTGGGCTTCCTTGATGTTCACCACAAATGCTTTCTTCTCGAAGTTGTACTCTAGAGACAAGAAGAGTGTCCCCAGCTTCTCTTGTAAGATTGTCATTTTGATGTACGGGTCAGAGGTCATGGATTGCTCGTCCATGGCTGGTAAGCCCTGGGCTTCCTTGATGTTCACCACAAATGCTTTCTTCTCGAAGTTGTACTCTAGAGACAAGAAGAGTGTCCCCAGCTTCTCT
Peptide sequence:
MYGSEVMDCSSMAGKPWASLMFTTNAFFSKLYSRDKKSVPSFSCKIVILMYGSEVMDCSSMAGKPWASLMFTTNAFFSKLYSRDKKSVPSFS
IRESfinder result
IRESfinder score
0.673182285
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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