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mmu_ciGls_825
Basic information of circular RNA
PanCircBase ID:
mmu_ciGls_825
mm39_Chr. Position ID:
chr1|52227012|52227837|-
Gene name:
Gls
Transcript:
ENSMUST00000114513.9
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
14
Exon/intron offsets:
0
Genomic length:
825
Exon/intron sizes in nt:
825
Flanking exon/introns:
chr1:52208117-52227837
Splice length:
825
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGCCAGTCCTTCCTTAGATCCTTCTCAGTGTCCTCTAAGATGAGCAAGCAGCCCTCCTGCTTACGTAAACGTCAGCTTGTTAACAGCCTCAAGTCTTTGCCAATTCCAAATAAGTTATTTTTATTGTCCGTCATAGATACTAAAATCATTTGTAATATTTAAACAAAATTGTTAGCTTAGCAGTGCTGGTTTTAAAGCTAAAGCCTTGTTTTTACTCCAAATATTTGTCAGTTATTGTTCGGGATTAAATTTAGCTCATTGTCTAGTTTGTGCATGGATATGTAAATAGATAGGTTCATAAGACTAAATAAGAAAAAGGCCACTGGAGATAACAGTTTTTCCAGAATGTTTAATGATATGATTTTGATGGTGGTAATAAGAAAGCTGTACTACTATATGCTAAATACCCGTAAGGTCTTGTTTATTATCACAGTGTGTGGCCCCATGTGCATAAACATTTCTTTTTTTTTTTAAGAGTTAGTCTTAAGATCAAGACTTGAGTTTGTTTTTTTCATTGGGCCAAAGGACTTGCTGTTGAAAATTTTAACTCTATTCTTTTACATCATAAAATTACCTTGTACACTAGAAACACACTTACAAATAGTATTTTTTAAATGTGCTAAGTACATTTTGTAAGTGCTAACTTAAATTTGTGTTAGTTCCAATCAGAGTAACCTTTACTAATATCTGAGAACTTTGAAATGCTGATAGTGTAGGAGCTTTCCTGCCAATCAACATGAGTAGCATGTCAAGATGTCAGACGATGATGGGTGAAGATGTTAACTGATCTTAATAACTTTCATTGAAAAATACATTTTATAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCAGACGATGATGGGTGA
58.44
TTTACGTAAGCAGGAGGGCTG
60.07
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GATGTCAGACGATGATGGGTGA
59.9
TTACGTAAGCAGGAGGGCTG
59.46
141
PCR template sequence:
TCCTGCCAATCAACATGAGTAGCATGTCAAGATGTCAGACGATGATGGGTGAAGATGTTAACTGATCTTAATAACTTTCATTGAAAAATACATTTTATAGGTAAGCCAGTCCTTCCTTAGATCCTTCTCAGTGTCCTCTAAGATGAGCAAGCAGCCCTCCTGCTTACGTAAACGTCAGCTTGTTAACAGCCTCAAGTCTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-196a-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATACATTTTATAGGTAAGCCAGTCCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.90265411
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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