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mmu_ciG6pc2_525
Basic information of circular RNA
PanCircBase ID:
mmu_ciG6pc2_525
mm39_Chr. Position ID:
chr2|69050594|69051119|+
Gene name:
G6pc2
Transcript:
ENSMUST00000005364.12
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
525
Exon/intron sizes in nt:
525
Flanking exon/introns:
chr2:69050594-69051126
Splice length:
525
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGACTTCTGTTTTATCTTTCCTGAAATTTATCCTTAAATTTGTAATCTTAGCTTAGTGATCACGTTTCAGGTGCATGTACTTCGACACAGAAATCTTCCAAACAAGCTGGTTACCTTGAAACACCAGATTCATAAGTAGAACTAACAGAAAGCATAGAAATATATACTTTGAATTACAAGTGATTTCACACTTAACACATATGCTATCATTAACCAAGAAGTTAGCAGTACAATAATGAGATTGATGTTTCTAAGTTCCATTTTGATAATTCCAAACGAAGTACCCAAAGCATCCACAGGACTTCCCTGGTTATGGGAGACAAGTATTTCCAAACCCCAGATACTACCATGCTCTTTCATGATGAGAGACCAGGACTCAAGTTATTTAAGGGTGTTTTAATTATGAGTTTTCACCCTTGACTCTTTACTTGAGGTGCGTGTTTTTTTTTTTTAAACAAGAATTGTGAGTCTCCACTTAGCCAGCTGTTGTATTTAAGTACATATGAAATTCATATTTGTTTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTCCACTTAGCCAGCTGT
59.39
TGTGTCGAAGTACATGCACCT
59.66
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCACTTAGCCAGCTGTTGT
59.24
TGTGTCGAAGTACATGCACCT
59.66
144
PCR template sequence:
CTTTACTTGAGGTGCGTGTTTTTTTTTTTTAAACAAGAATTGTGAGTCTCCACTTAGCCAGCTGTTGTATTTAAGTACATATGAAATTCATATTTGTTTAGTAAGACTTCTGTTTTATCTTTCCTGAAATTTATCCTTAAATTTGTAATCTTAGCTTAGTGATCACGTTTCAGGTGCATGTACTTCGACACAGAAATCTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-224-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCATATTTGTTTAGTAAGACTTCTGT
siRNA sets
ACAGAAGTCTTACTAAACA, TCTTACTAAACAAATATGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.950784208
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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