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mmu_ciFus_1262

Basic information of circular RNA
PanCircBase ID:

mmu_ciFus_1262

mm39_Chr. Position ID:

chr7|127575092|127576354|+

Gene name:

Fus

Transcript:

ENSMUST00000106251.10

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

8

Exon/intron offsets:

0

Genomic length:

1262

Exon/intron sizes in nt:

1262

Flanking exon/introns:

chr7:127575092-127576389

Splice length:

1262

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAAGCCCATGGTTTTTATATAGTATTCACTTGGATTTTCTTAGAAACAAAAAATACTGCAAAAGTGGAGAGTGATGGGAGGGGCTGTATAAATGCTAGAAACCCTGGATCTGGAAGTTTAGACTTCAGATTCTGGTATTTATTATTTCTAGTACGTCGTGGGTCTTGCTTGGGAGCTGTTCTTTTAAAGTTGATGGTGTTTTAAAGTCCAAAAATTACTAAACCAGACAATTTTTTACTTATTTTTTTGGATTTTTGAGATGGATTTCTCTGTATGACTCAGAAATCCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGCGCCACCACACCCGGACAGACATGATGTTTCGTACCTTTATTCCTAACAGGATGATCTCTTGAGTTCATGTCTAGGCCATCCTGCTCTACAGAGCTAGTTCCACGACAGCCTGGGCTATACAGAGAAACTAAACTCAGTTTTAAAAACCAAAACAAAGAAATGCACACATGTAAACTGTTGAGTAACATTAGGTGGATAAAAAGACCAGACTGAAAGATGTGTTAAGGGAACCACTTTACTAGATGTTTTTTGATGGTATTCCTATAGGCTATACTATATTTAAATGTGAAATTGGTCTCATTCCTTTCTTTTGATTTTTAGTTACAGTCATCTACATAATTATTTTGAGGCAGGATCTCTACATAGCCTTGGCTGTCTTGGAACTATGTAGACCAGGCTGTTTTTAGAATTCAGAGATCTGGGGCTGGAGAGATGGCTCAGTGGTTAAGAATACTGACTGCTCTTCCAGAGGTCCTGAGTTCTAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGGGATCTGATGCCCTCTTCTGATGTGTCTGAAGACAGCAGACAGTGCTCACATACAGAATTAATTAAAAAAAAAAAATTCAGAGATCCACTTGTCTCTGCATCCAGAGTGCTGATATTAAAGGAGTGTTCCACTATGCCCTGGCATAAAAAGTTTTTTTTTTTAAACTAAGGAGTCTAGAAATGAAAGTTTGGAAACTGGTTTTTATATTGGGTGTTGGTGTTTCAAGGACAGTAATGAGTAAATGTAAAATTTTTGAAGGAAGTAGATAATACTTAAATATGGATTGTTTTTGATTATCCAATAAGATACTGTGTAACTGGTAAGAATCCTGAGCATTTTTCTTGAAAGCTTTAAAAGTACACATATATGGTTGGTATACCTGGCTAACTTGTCTTTCCGTTTTTTCTT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

ACTGGTAAGAATCCTGAGCA

55.85

CCCTCCCATCACTCTCCACT

60.33

176

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TCCTGAGCATTTTTCTTGAAAGCT

59.41

ATACAGCCCCTCCCATCACT

60.03

172

PCR template sequence:

CTGTGTAACTGGTAAGAATCCTGAGCATTTTTCTTGAAAGCTTTAAAAGTACACATATATGGTTGGTATACCTGGCTAACTTGTCTTTCCGTTTTTTCTTGTAAGCCCATGGTTTTTATATAGTATTCACTTGGATTTTCTTAGAAACAAAAAATACTGCAAAAGTGGAGAGTGATGGGAGGGGCTGTATAAATGCTAGA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

5

Interacting miRNAs (#binding sites)

mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-297a-5p(1), mmu-miR-1187(1), mmu-miR-5101(1)

siRNAs for circular RNA silencing
Junction sequence

TCCGTTTTTTCTTGTAAGCCCATGGT

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.855073552

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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