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mmu_ciFbh1_802
Basic information of circular RNA
PanCircBase ID:
mmu_ciFbh1_802
mm39_Chr. Position ID:
chr2|11760610|11761412|-
Gene name:
Fbh1
Transcript:
ENSMUST00000131893.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
802
Exon/intron sizes in nt:
802
Flanking exon/introns:
chr2:11760588-11761412
Splice length:
802
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGGGCAGCTTTCCTGTGTCCAGTAGAAGATGTCCTGGGTCGGCTAGGTGGAACGGCTTTGCTTAGGAGTTGTGACTCTTGGTTCCTACTTCTTCTAGGCTATTTGAAACTCTGGCAGCTGAGCAAACCTTTGCTTGCCTCTTTTGACGCCATCTTTGTGGATGAGGCCCAGGACTGTACCCCAGGTGATAAACTATTCAGGTCGTCAGTAGTCTCAAATCCGTAGAGACAGCGTTATGATTATGTAAGAGGACATACACATGGAATAAGTCATTCTTATTTGTGATGAGGAGAGGAGTGAATGATTCTTAGTCTTTCAGAAATTAGACCCAAAGTTCCCACAGCTGTCTGTTCCTCGCCTGAAGTACAGTTGTCCTCTTCCTCCGTATGCTTTACTAAGGTTGGTCTTAAGTTGCTGAGCAGCCGGCTCATCAGTCACAGGAACCTAGCCTCATGCTGAGTGAGAAACGTGGTCAATTTAGACTGAAATTTTATGCTGCATGACTAATAGGGAATATAGACAGTATTAAAATTTTAACTTTCTAGGTATTTTGGAGATGTTATGCTATTAGAATGGAAATTTTTATTTGCTTATATATTTTCTTATGATCAGTTCACCCAGGAATAAAATTAATCTGCGTCCCTAGGACCAGATACCATTACTGTGCCCATGGTAAAATAGAAAGCCTGCCCTGTGGAAAAGAAATTATTGTCTGTCGTGGGCTGGCTTAGTTTATGTTTGTTTTCTTTCTTAGATATGGCTTATTTTCGCTGCATGGATTGTTGCCATTCAGATTTCTCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TATTGTCTGTCGTGGGCTGG
59.75
AAGCAAAGCCGTTCCACCTA
59.89
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TATTGTCTGTCGTGGGCTGG
59.75
TAAGCAAAGCCGTTCCACCT
59.89
160
PCR template sequence:
GAAATTATTGTCTGTCGTGGGCTGGCTTAGTTTATGTTTGTTTTCTTTCTTAGATATGGCTTATTTTCGCTGCATGGATTGTTGCCATTCAGATTTCTCAGTGGGCAGCTTTCCTGTGTCCAGTAGAAGATGTCCTGGGTCGGCTAGGTGGAACGGCTTTGCTTAGGAGTTGTGACTCTTGGTTCCTACTTCTTCTAGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-495-3p(1), mmu-miR-22-3p(1), mmu-miR-466a-5p(1), mmu-miR-466p-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCAGATTTCTCAGTGGGCAGCTTTC
siRNA sets
AAAGCTGCCCACTGAGAAA, CTGCCCACTGAGAAATCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIFBH1_802_ORF_3
0.9926
-0.238903961
0.026327504
111
ORF sequence:
ATGGCTTATTTTCGCTGCATGGATTGTTGCCATTCAGATTTCTCAGTGGGCAGCTTTCCTGTGTCCAGTAGAAGATGTCCTGGGTCGGCTAGGTGGAACGGCTTTGCTTAG
Peptide sequence:
MAYFRCMDCCHSDFSVGSFPVSSRRCPGSARWNGFA
IRESfinder result
IRESfinder score
0.784158176
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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