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mmu_ciFam214b_106
Basic information of circular RNA
PanCircBase ID:
mmu_ciFam214b_106
mm39_Chr. Position ID:
chr4|43039859|43039965|-
Gene name:
Fam214b
Transcript:
ENSMUST00000107958.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
106
Exon/intron sizes in nt:
106
Flanking exon/introns:
chr4:43037206-43039965
Splice length:
106
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTGTGTACTAGGTGGAAGGATCAGATCTCCAAAACCTCTGCAGGGTATGGGGCCTGTCCCAGACTGTGAAGAAGGGATGAGAACCGACCTGGGAGGCTAAGC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGCTAAGCGTGAGTGTGT
60.04
ACCCTGCAGAGGTTTTGGAG
59.89
60
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTGTCCCAGACTGTGAAG
60.32
ACACACTCACGCTTAGCCTC
60.04
61
PCR template sequence:
GGGCCTGTCCCAGACTGTGAAGAAGGGATGAGAACCGACCTGGGAGGCTAAGCGTGAGTGTGTACTAGGTGGAAGGATCAGATCTCCAAAACCTCTGCAGGGTATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-27a-3p(1), mmu-miR-298-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGGAGGCTAAGCGTGAGTGTGTACT
siRNA sets
TACACACTCACGCTTAGCC, GTACACACTCACGCTTAGC, ACACACTCACGCTTAGCCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.411638638
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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