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mmu_ciFam135a_992
Basic information of circular RNA
PanCircBase ID:
mmu_ciFam135a_992
mm39_Chr. Position ID:
chr1|24124608|24125600|-
Gene name:
Fam135a
Transcript:
ENSMUST00000187752.7
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
992
Exon/intron sizes in nt:
992
Flanking exon/introns:
chr1:24124607-24125600
Splice length:
992
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGCATATGGCATTTCCTTTGCACTCACTTCATCATAGGTTAGAATACTGGCTGAATTTTGGCAAGTTTTTCGAGAGCCGTGAAGGCTGAGAGATTTTACTGTGCTGGCTAATGAATGAGTTGCTTGCTATGGTGCCATAATGTTGGCAGGAGACATTGGACTTAACAGTCAGAGACAAAGATGGGTTCTACTCAGAGCAGTCGGGCCAGGTGACATCACATAGCAACAAAGTATAGAAGAGGAGAGGAACCTGAGCTAAAGAATCCTGGAACTTTGATGGTGGACAGTCCACCCACCTGTTTCCTGTGTGGGAAGGCTTATTGTGTAGAATAGTAGCAGGTCTCTTCTTAGCTCTAGTGTGAGGCACTGGTGCTGGCTTTTCAGATGCCCATGAAGAGATAGCCTGGGACAAAGGCAACTGTTAGAGTCCTTGCCTTAGTAAGAGGTGCAGAGATACCAGAAATCAGTGAGCATTATCTCCAAGCAATTGTCGTCTCTAACTTGCTCTTCCAAATATGTGCTCTTGGTATGGATGTTTGTTATCTGTCTAAGTAGTGAAAATAGAAACCACTGACACCAGTTTTATACACACACACACACACACACACACACACACACACACGGAACTATTTTTCAATGCGTATGTGAATGTTTTAATTTTCACTTGTTTGTGCACCTTTTATTTTAACTTCCATGAAAATTAATGCCTTAGGTTTTTATGAGAGGAATTTTTTTTTAATTCAAAAGTGCTTTGTTCTTTAAAAAATATTTTCTTCATTTAACAAAAATATCCATTCATGGGTTATCATATGGATGGCATAGGGGAAGAAATGTTAGAAAAGCTTTATAACCAACACATCTTGTGGTAAAAGTAGCACTGCTGGTTGGTATCTCTGGATTCTGGTCCTGCTTGGTTGGATTAAGCTAAGCTAGGCTTTTAAGTTGGCATTTCTGTTGTGTTTCTCATGATCTCTCCCCCTTTTAACTAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTGGTCCTGCTTGGTTGGAT
59.96
CGGCTCTCGAAAAACTTGCC
60.11
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCTGGTCCTGCTTGGTTGG
60.18
CGGCTCTCGAAAAACTTGCC
60.11
173
PCR template sequence:
ATCTCTGGATTCTGGTCCTGCTTGGTTGGATTAAGCTAAGCTAGGCTTTTAAGTTGGCATTTCTGTTGTGTTTCTCATGATCTCTCCCCCTTTTAACTAAGTAAGCATATGGCATTTCCTTTGCACTCACTTCATCATAGGTTAGAATACTGGCTGAATTTTGGCAAGTTTTTCGAGAGCCGTGAAGGCTGAGAGATTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-1187(2), mmu-miR-574-5p(2), mmu-miR-155-5p(1), mmu-miR-335-5p(1), mmu-miR-18a-5p(1), mmu-miR-495-3p(1), mmu-miR-130b-3p(1), mmu-miR-301b-3p(1), mmu-miR-183-5p(1), mmu-miR-301a-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCCTTTTAACTAAGTAAGCATATGGC
siRNA sets
TGCTTACTTAGTTAAAAGG,ATGCTTACTTAGTTAAAAG,GCTTACTTAGTTAAAAGGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.818707485
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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