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mmu_ciEro1b_2295
Basic information of circular RNA
PanCircBase ID:
mmu_ciEro1b_2295
mm39_Chr. Position ID:
chr13|12619350|12621645|+
Gene name:
Ero1b
Transcript:
ENSMUST00000071973.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
15
Exon/intron offsets:
0
Genomic length:
2295
Exon/intron sizes in nt:
2295
Flanking exon/introns:
chr13:12619350-12621715
Splice length:
2295
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGCGGAGACCTGATTCCCTCCAGGTTTCAACATACGGCAAAGGTTATCTTTCCCCCAAAGGTATGCCTTGGTTTGGTTTGCTGACAGATAAATGCTGGCTGTGGTGGCACACACTCATAACCGCAGCACTGAGAAGTTGAGGCAGACAGACCATGAGTTCAAGGCTAGCTATCATGGCCCATGTAGTAAGGACCTTGTTTCAAATAAACACCTTAAATTCATTCTTCAGTGTGGCCTCCAGGCTGATAGGCAAGTTCTCTCCATCAGTCCCAGGAAGAACTGAGAGTTTGCAGCTATAGTGCTTTTCAATTTTCTTTCTCAATTTCATGAGGAAAATAGCCTCCGTGTGTGTGTGTATAATTATCAGAAATGTTTTTTTAAGTAATTTGAATTTATCTACAGAAATATATGCAGTGTTGGGATATAAAGCAGAAGGAAAAGAATCATAGAAAATAATATTCTGTGTTGTAACAGTAGAACAAAATAAGTCAAATCACAGGGGAAAATTTATTTTTAAAACAGGACCAGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAAAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCATAACCATCCTTAATGAGATCTGACGCCCTCTTCTGGTGCATCTGAAGACAGCTACAGTGTACTTAGATATAATAGTAAATAAATCTTTAAAAAAAAAAAAACCAGGACCAAATGACAGAGATTAATATGTATGTTTTATGAGTGTGATTTTTAAGTTCAAAGGAGACCAATCACGCAAAGATGACTTAGCTATGCCCCAGCGTAATGCCAAAGCCTTCACCGAGTTCCTCCATGTGCTCAGTTTTGTCCAGGCAGGGTTTTGTTCTAGTGGAGCCTGACCAGCTCACTGAGCCTGCCTACCCCAAGCCCTAGGTGAACTGACTGCTAGTCCAGCTCTTGTCTGTACTTAAAGATGTCTGTGCTAATTGACATACAAACCACAAGGCAGATAGCATCCTGATTACGTTTTTATATTTCAGGAAATATCAGTTTATGCATTTTTTGAGGTCTGAAAAAAAATAATAAGGACGAGGCGAGGTGAAACGTAGATTATTTGAAATGAAAAAGATGTTAAATAACTTAGTTTAAGCACAAACAAAATTAAATTGAGAAAGGAAAATTTTAATTGAATATTTCATTAAAACGCCATTGGCTTTCATAAATGCTCCCTTTTAGCCTCAGCCTGTGTCTAGAGCCCATTCTCCCTCCAAGGGGCGCTGGACAGGAAGCAAGTCCTAGAGTCCTGGGATCCTGGCCTGCTCCTTGCCAGCGGTTCAGCTCGGCAAACCTTGGCTCCACACCCAGAACCCATTGAGACCTGGGGGATCCTGGAAAAAGATTTGATCCTAATTTTACCTAAAAATGTAAAAGCTGGGCTGGTAAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGTCCTGAGTTCAAATCCCAGAACCACATGATGGCTCAAAACCATCCATAACAAGATCTGACTCCCTCTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACATATAAATCAATCAATCAATCTATCTATCTATCTATCTTTTTAAAAAATTAAAAGCTCAGCTGTAGCTAACTTCTGTTGTGTTCTTTGAGTTTGTTGCGCGTGTATACTTGACAGGTGGACACTGTGGCTAGGCTGTAGAAGAAATAGCAGGTTCGTTAGGTTAACCCTGAGTTCATAAGGGAGAGGAACTTGTAGGTTCCCATTCTTCCAACCCCAGGGTTTGATGTCTGTGTTTGAGCTCTTTATTTATTCATTGAGCCAGTAATTACTTGTACAATGCCATAAGTATAGACGGGTACTGGGAGTGGGAGGGACAGTGTAGACAGTGGCACTGTGTCCATAGTTCATCTATAGGCTTTTTGTGGTTGTTTCATTTTTCAAGACAACGTTTCATTGTGTAACCCTGGCAAGTCCTGTAACTTACTTTGTTGACCAGGCTGGCCCCAAACTCAAGAGATCGGTCTGCTTCTGCCTTAGTGCTGTAGTCAAAGGTGTGTATGTACCACACACCTGCCTCTCATGGATGGTCTCAGTGTGAAGACAGAGTGGTTTCTGTTGCCCTTTTAAGCATGCCCAAAGGCCCACAGCTCCATGACCTGTCAGCTTTAGTAGGAATCACAGCATTTCTCTTTTAAATGGAATTGTCAGCATTCAGTGTTTTGGTTCTTTTAGATTCTTTGCTTCCAGGTTATATAGCTCAAGGAAGCTGTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCAGCATTCAGTGTTTTGGT
58.98
CAGCAAACCAAACCAAGGCA
59.82
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCAGCATTCAGTGTTTTGGT
58.98
TCAGCAAACCAAACCAAGGC
59.54
156
PCR template sequence:
GGAATCACAGCATTTCTCTTTTAAATGGAATTGTCAGCATTCAGTGTTTTGGTTCTTTTAGATTCTTTGCTTCCAGGTTATATAGCTCAAGGAAGCTGTAGTAAGCGGAGACCTGATTCCCTCCAGGTTTCAACATACGGCAAAGGTTATCTTTCCCCCAAAGGTATGCCTTGGTTTGGTTTGCTGACAGATAAATGCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-128-3p(3), mmu-miR-3065-5p(2), mmu-miR-466b-3p(2), mmu-miR-490-3p(2), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-let-7e-5p(1), mmu-miR-223-3p(1), mmu-miR-31-5p(1), mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-24-3p(1), mmu-miR-501-3p(1), mmu-miR-96-5p(1), mmu-miR-218-5p(1), mmu-miR-297a-5p(1), mmu-miR-466d-3p(1), mmu-miR-466a-5p(1), mmu-miR-466p-5p(1), mmu-miR-5101(1), mmu-miR-484(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAGGAAGCTGTAGTAAGCGGAGACC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIERO1B_2295_ORF_1
0.5729
-0.239358237
0.024524522
219
ORF sequence:
ATGGAATTGTCAGCATTCAGTGTTTTGGTTCTTTTAGATTCTTTGCTTCCAGGTTATATAGCTCAAGGAAGCTGTAGTAAGCGGAGACCTGATTCCCTCCAGGTTTCAACATACGGCAAAGGTTATCTTTCCCCCAAAGGTATGCCTTGGTTTGGTTTGCTGACAGATAAATGCTGGCTGTGGTGGCACACACTCATAACCGCAGCACTGAGAAGTTGA
Peptide sequence:
MELSAFSVLVLLDSLLPGYIAQGSCSKRRPDSLQVSTYGKGYLSPKGMPWFGLLTDKCWLWWHTLITAALRS
IRESfinder result
IRESfinder score
0.801045364
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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