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mmu_ciEola1_704
Basic information of circular RNA
PanCircBase ID:
mmu_ciEola1_704
mm39_Chr. Position ID:
chrX|69431772|69432476|+
Gene name:
Eola1
Transcript:
ENSMUST00000053981.6
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
704
Exon/intron sizes in nt:
704
Flanking exon/introns:
chrX:69431772-69432477
Splice length:
704
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGTGAGGCTGTACCAAATACCACGTAGACAATTCCAACAGGATCCTGCTTGATAGATTGGTGGCACTGTTTTGCTGTTAGTTAGAGGGCGTGTCCTAAAGTTTCCAAGAAGACTCACTAAGGACTACCAAAGTCACTTGAATGAGGTGAATAGGTCTGGGTTCCACAGACTCAGTTACGTCTTAACTCTAGGCCTCATGTAGCATTAGACTGTGTGTGCATGAATATGTGCAAAAGGCATGGTCTATGACAGCATAGACACTGAAAAAGTGGAGCATTAATAGAGTAAGGCTGCTTGCCTGGCTTGTAATGCCTCTCACAAGAACCTTGAGTAAGACACCAATCAACATCTAATACGACTAGTCACCTACTGACCTAATTGGGAATCTTATTGACTGTGGGCTTCCTGGATAAGACTGGTTCTCTTTCAGTAAATGCCAGCCTTAGTAGTATGCTAGAACCTGACTGTTCTTTAATGCAGGACTCACGGAGCATAAGCCACCCTCTTCTTTTTCCCAAAGCCTGTGGCCGTCTCACCCAGGATTGCCTAGTTCAGTTGGTCATGTTTCCAATGTTCAGTGAGAAAAATCCCAATTTCCTATCAATCATTTTGATTATACACTATCAGTTGACCCTGCAGTCATAAAGATAACCTTGTTGTATAGAAGAGACTACATCTGTATATTTCTGTCTTTGTGCCCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGTTGACCCTGCAGTCAT
58.94
CAGCAAAACAGTGCCACCAA
59.82
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATCAGTTGACCCTGCAGTCA
58.94
CAGCAAAACAGTGCCACCAA
59.82
158
PCR template sequence:
CAATCATTTTGATTATACACTATCAGTTGACCCTGCAGTCATAAAGATAACCTTGTTGTATAGAAGAGACTACATCTGTATATTTCTGTCTTTGTGCCCAGTAAGTGAGGCTGTACCAAATACCACGTAGACAATTCCAACAGGATCCTGCTTGATAGATTGGTGGCACTGTTTTGCTGTTAGTTAGAGGGCGTGTCCTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-155-5p(1), mmu-miR-27a-3p(1), mmu-miR-222-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCTTTGTGCCCAGTAAGTGAGGCTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.82108173
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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