top of page
mmu_ciEif4g1_179
Basic information of circular RNA
PanCircBase ID:
mmu_ciEif4g1_179
mm39_Chr. Position ID:
chr16|20502931|20503110|+
Gene name:
Eif4g1
Transcript:
ENSMUST00000044783.14
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
20
Exon/intron offsets:
0
Genomic length:
179
Exon/intron sizes in nt:
179
Flanking exon/introns:
chr16:20502931-20503157
Splice length:
179
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTGTGAGGCTCGGGCTGAGGAGGCAGAGGAGCTCCTAGAGGATATAATACCCTTTCCGCTGATGACTTCTGTCAGTGCCACGTGTCTGGGCCACTGAGACCACATGATGGGATTGAGGACCTGAGGAAGAGAGGCTAAGGGTGGCCCAAGAAGGGGTTGTCAGGACTTGTTACTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGAGAGGCTAAGGGTGGC
59.82
ACGTGGCACTGACAGAAGTC
60.25
137
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCACTGAGACCACATGAT
59.75
TCTAGGAGCTCCTCTGCCTC
59.82
130
PCR template sequence:
TGGGCCACTGAGACCACATGATGGGATTGAGGACCTGAGGAAGAGAGGCTAAGGGTGGCCCAAGAAGGGGTTGTCAGGACTTGTTACTCGTGAGTGTGAGGCTCGGGCTGAGGAGGCAGAGGAGCTCCTAGAGGATATAATACCCTTTCCGCTGATGACTTCTGTCAGTGCCACGTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-183-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGACTTGTTACTCGTGAGTGTGAGGC
siRNA sets
ACACTCACGAGTAACAAGT, ACTCACGAGTAACAAGTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.3471134
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page