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mmu_ciEif4a1_288
Basic information of circular RNA
PanCircBase ID:
mmu_ciEif4a1_288
mm39_Chr. Position ID:
chr11|69559615|69559903|-
Gene name:
Eif4a1
Transcript:
ENSMUST00000128519.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
5
Exon/intron offsets:
0
Genomic length:
288
Exon/intron sizes in nt:
288
Flanking exon/introns:
chr11:69559579-69559903
Splice length:
288
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTACCATGTAACAGTTGGTGGGCATTCCCACATGTCATTTTCTTGGATGCAAGTTTTCCTTTAGTTTAGAACTGAGTTGCTCTGTGATGAACCCCATGCGTGTCATCTGAGCCTGGCTTCCCTGTTGTTACCTTAGCCCAGGTAGTGTTCTACTTACCAGGGCTTTTGCCTGGCTTATCAGGGAAGCTTTGGGCAAAGGATCAGTCCTTGTATTCTGAGAGCAGACATCTGCCCTTTGACTGCCCTCTTACCAAGAGATTTCCATGTATTTCACATTTGTCTGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCTTTGACTGCCCTCTTA
59.67
TGGGGTTCATCACAGAGCAA
59.23
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACATCTGCCCTTTGACTGCC
60.61
TGGGGTTCATCACAGAGCAA
59.23
160
PCR template sequence:
AGCTTTGGGCAAAGGATCAGTCCTTGTATTCTGAGAGCAGACATCTGCCCTTTGACTGCCCTCTTACCAAGAGATTTCCATGTATTTCACATTTGTCTGAGTGAGTACCATGTAACAGTTGGTGGGCATTCCCACATGTCATTTTCTTGGATGCAAGTTTTCCTTTAGTTTAGAACTGAGTTGCTCTGTGATGAACCCCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-141-3p(1), mmu-miR-381-3p(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
CACATTTGTCTGAGTGAGTACCATGT
siRNA sets
ACATGGTACTCACTCAGAC, ATGGTACTCACTCAGACAA, TACTCACTCAGACAAATGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.834692926
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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