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mmu_ciEif2b1_1191
Basic information of circular RNA
PanCircBase ID:
mmu_ciEif2b1_1191
mm39_Chr. Position ID:
chr5|124709949|124711140|-
Gene name:
Eif2b1
Transcript:
ENSMUST00000031334.15
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
7
Exon/intron offsets:
0
Genomic length:
1191
Exon/intron sizes in nt:
1191
Flanking exon/introns:
chr5:124709949-124711140
Splice length:
1191
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAGGTACTGTCATACTGTTCCCAGAAGCCCCTACAGCCTGAGCAAGACCTTGTCAGCTTTCTTCTTTGGAGGCTGATCTTGATGAATTTAGTCAGTAGCATTTACATGTATACACAATAGGTACTTTTGTTTGGATATGTAACTTGAGACCAGGTATTATGTAGCTCAGGATAGCCTCAAATTGTCTCAAACTCCTGTCTGTGCCTCCCAAGTGCTGAGATCATAGGCATGACCATCACACCCAGGCAAACTGGATTCAGCTTGGAATTGAGTGTCTCTGGTTTTGTTTCCATTTGATTGAGGACTTCCATCCCCAGGGTTTATTACATTTTCTCTGTCACTTACCTTGGAAATTATGACAGATGTCATAATCATATTAAGCTGTGCATGGACCGTCATAAGTCTCAGGTTCACTGGCATTCGGCACACTCAAGCCATGCAATTGCCAGTATTTACAAAGCTTTCTATTTGTCCCAGATGGCCTTTTCCCATGTCCCTGGTCACCTTTGTTCTGCTCTGTCCTGATTTTTCCAGGCGAGGGAACCACAGATAAGTAGATTTACATGACACTTATGATTTTGAGCTTGACAAACTTCACTGGCCCCACTGCAGCATGTGTCATTGCTGTGTGCTTTCAAGCAGAGTACCGTTCCATTATAACACGACCACTTGCCCATGTTCACCTGTAGTGGACACTTGGATTTTCCTACCGTTGGCTGTGGTGCAGGGTGCAACTGTAGACACTGGTCCAGTGTCTGAGGCCCCCAAATATGGAAAGTGCTGGCTTATCTTAGACTGCACTTACTTTTTCTAACTGGTGATGCTGGCATCTTTTCATGTGCTTTGGGAACATTTATCTTTCTTTCTTAAAAGGTCCTCTTTGAAGGCTAGGAATACAGCTTAGCTGGTAGAATGCCTGTCTAGCACACTGAAACCCTTAGTTCAAGCCCCAGCACTGAATAGACTAGGCCTGGTGGAACTCAAGAAATCCCAGCACTAGGTGGTGTGGAGGCATGAGGATCAGAAGTTCAAAGACATACTCGAGTGCAAGGCCAGCATAAAATACATTAAGACCTTGCCTTTTAAAAAGCAACAGTTCAGATCGCCACGCCAAGATTAAACTTACTGTGTTTCCTTCTGTTTCACTGAGCTATGCAAGCTCACCCTGTCCATCTTTCCTGACTTCTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAACAGTTCAGATCGCCACG
59.56
GACAAGGTCTTGCTCAGGCT
59.96
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGATCGCCACGCCAAGATTA
59.54
GACAAGGTCTTGCTCAGGCT
59.96
146
PCR template sequence:
CAACAGTTCAGATCGCCACGCCAAGATTAAACTTACTGTGTTTCCTTCTGTTTCACTGAGCTATGCAAGCTCACCCTGTCCATCTTTCCTGACTTCTCAGGTAGGTACTGTCATACTGTTCCCAGAAGCCCCTACAGCCTGAGCAAGACCTTGTCAGCTTTCTTCTTTGGAGGCTGATCTTGATGAATTTAGTCAGTAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-20a-5p(1), mmu-miR-221-3p(1), mmu-miR-139-5p(1), mmu-miR-24-3p(1), mmu-miR-431-5p(1), mmu-miR-153-3p(1), mmu-miR-369-3p(1), mmu-miR-669d-5p(1), mmu-miR-3065-5p(1), mmu-miR-574-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTGACTTCTCAGGTAGGTACTGTCA
siRNA sets
AGTACCTACCTGAGAAGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.806323279
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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