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mmu_ciE4f1_391
Basic information of circular RNA
PanCircBase ID:
mmu_ciE4f1_391
mm39_Chr. Position ID:
chr17|24664528|24664919|-
Gene name:
E4f1
Transcript:
ENSMUST00000056032.9
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
8
Exon/intron offsets:
0
Genomic length:
391
Exon/intron sizes in nt:
391
Flanking exon/introns:
chr17:24664525-24664919
Splice length:
391
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAGGTTTCTGTTACTGTCTTGCTCCCTCTGACGGGGCTGACCCAATGTTTGCTTCTCATGTGGGGGTGGGAGCTGCTAGCTGCCTCATTCATTTTTATTTAGTTTTTTTCTGTCTTTGAGAAACAGTCTAGTGTAATCCAGGTAGCCTCAAATTTACAGTGTAGCCAGTGTTAACCTCAGTTCTTGGTTCTCCTGCTTCTACTGGGTACTGGAATTGCAGGCTGCACCACCATGCCTGGCTTGAAGAGTAGCATTTCTTTCTGCTTTACTGCTCTCTAGACTGTTAACTCCCTTACCTAAAGCCTGGAGGGAAGAGGGCTGACTGTTGCTTTTGGTACCATTGAAGCGTAGCTTACAGTAGGTACTAGAGGTTGGTCTGTGGTCTCCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGCTGACTGTTGCTTTTGG
59.97
CACCCCCACATGAGAAGCAA
60.25
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGAGGGCTGACTGTTGCT
59.96
CACCCCCACATGAGAAGCAA
60.25
149
PCR template sequence:
CCTTACCTAAAGCCTGGAGGGAAGAGGGCTGACTGTTGCTTTTGGTACCATTGAAGCGTAGCTTACAGTAGGTACTAGAGGTTGGTCTGTGGTCTCCCAGGTAGGTTTCTGTTACTGTCTTGCTCCCTCTGACGGGGCTGACCCAATGTTTGCTTCTCATGTGGGGGTGGGAGCTGCTAGCTGCCTCATTCATTTTTATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-141-3p(1), mmu-miR-132-3p(1), mmu-miR-194-5p(1), mmu-miR-802-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTGGTCTCCCAGGTAGGTTTCTGTT
siRNA sets
AACAGAAACCTACCTGGGA, ACAGAAACCTACCTGGGAG, GAAACCTACCTGGGAGACC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.927627905
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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