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mmu_ciDnmt1_2318

Basic information of circular RNA
PanCircBase ID:

mmu_ciDnmt1_2318

mm39_Chr. Position ID:

chr9|20843496|20845814|-

Gene name:

Dnmt1

Transcript:

ENSMUST00000178110.3

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

9

Exon/intron offsets:

0

Genomic length:

2318

Exon/intron sizes in nt:

2318

Flanking exon/introns:

chr9:20843495-20845814

Splice length:

2318

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAAGTGCAGCTGTCATGCCTCCGTGCCCTAAAGTGGTGCTTGCTTGCAGTGTCGCTGCTGTGAACAGGGGCCTGGGACTGAGCACTAGTGTATTTTGTAGCCTGAGAAGTTCCACTGAGAAAACCTAGACCAAGGTGGGCTGGAGAGATGGCTCAGCAGTTGAGAACTCTAGCTCCTTTTGTAAAAGATGCTGGGTTTTGTTCCTGGCATGTGGAAGCCAACTGCTGCCTGTAACTCCAGATCCCAGGGGGTTACTCCTCCGGCACATGTATACAGATGACATGCTGGCGGAACACCCATATACATAAAATACGGATAGCTGAAGAACAGACCCAAGGACCTCACCCAGTGAGGTGGCTCACTGGGTAAAGGTGCTTGTTCGCAAGTCTGACAGTTGAGCTCAACCCTGGAGCCTGCATGATGAAGGTTGTCTTCTGACCTCCATATGTGTCTATACTTGTACATACAGATAACTAAAGTCCAACCAAGCTGGCCATGGTGAGACACATCTGGAACCTCACCTTGTGTTGGAGGCTAAGGCAGGAAGGTTCTCATGAGTTCAAGGCCAGCCTGAGCTAGAGTGAGCCACTGTTTCAAAAAGAAATGAGAAACCATTAAGAAATAAGAGCATTGTGGTGCACACCTTTAATCCTAGCACTCAGGACACAGGTGTATCTCTGAGTTCTGAGTCAACCAGGTTTCAGGACAGCCAGGACTATAGTGAGACACCCTGCTTCAACAACAAAAGGTAGTCTGCCTACCCTAGAAAAGGGTAGCCTGCGACCTTCTCCTTACTCAGACCCTGCCTCAGCAGTCACGTTGCCATCCATCACGTTACTAGTGGCTGTTTAAGTGAGCTTGTGCTCCAAGTGTCTTGGCTGACGGTGTGAAAGCTTAGCAGCCACTGAGCTCGTCATTGAGCCTTGCACCTGGCAGCTGATTCTGGCTGACAGTGGGCTAACTTAATTGTCCACCACTTAGTCCACATGAAGACATCCTTATGTCCATAGAAGGGCACAAGCAACTAGTGCTGGCCTTCAATACTAAGTTTTGATTCTGGTGTTTGTATATATTTTTTGTTTTCGAGACAGGGCTTCTCTATGTAGTCCTGGCTGCCCTGGAACTCGCTCTGTAGATCAAACTGGCTTTGAACTTTGAGCAGAGATTTGCCTGCTTCTGCCTCCCGAGTGCTAGAGATAATGCTGTGCACCACGACCTGGCTTATTTGGAGGTTTTTAATAGATAGGATCTCCTATGTAATTCAGACTTGATCTCTGCTTCAGGATCAAGAATACAAGAATTTCAGGCCTGTTCTATCACATCCAGATAGAGATGATCCTCGTGACATGTCTTAACATGTGGGTGTCTCGAATCCAGACAGGGGATTTCTTACTGGCATGCATCTATGAGTTCAGATGGGAGTCCCAATTGACTCTGGAGGTCACACTAGGGCTAGACTCACACCTCCTAAGCACTGGAGTCACAGGCCCTGCCCAGTCAGTGCTTATGTAGGTGCTGGGCAGTCTGAACTTCAGTCCTTTTCTGAGCTAGTCCAGTCTTGAGATGGTATCGGTGGTGGTTTTCGGGTTTTTTGTTGTTGTTTTTGTTTTTAAAGCTTCTGTTAACACTCACTTGACTCATGCAAATATTGAAGGCCCAAGTGCAGAGGGCAGCCTGGTGCCTGATCCACAGATGCGCAGGGTGGAGGGTAGGGTGCAGAAGAGTAAGACAGTCGGGCTCCTGTGGGATGGAGGCCAGCCTTCTGTGCTTGCTGATGCTGGCCTCGCCACTCATTTCATCACGACAGTCCTGTCTGCCTCCAGGACCGCCAGTGAAGGCCTGCTAGTTGTTTGTCCTAACCTTTGGCATCTCTGATTATCGTTCATTTGAAGAGCTAAGTGGTGTGGTTGATAGACTCGGGGTGTGCACGGGCGCACACCAGTGTGTTTATCTGGTGTATACCCATGCTCCATCTTGCCTGCAGAGGTAAGGGGAGAGCTCCCAGGTTTCACTTCACTCCTCCCATTGTGCATTGTGGGGATGGAACTCGGATCATCAGGCCTACGTGGTAAAATCTTTCCTCTGTTCAGACGTCTGGTGGGCCCCAGCTGAGTGCTTGTTATCGGCTCATGAATGCTGTAGGCTTGAGCCCCAGTTCTGCAAAATGCATAAAATCATGAAGGATAAACAGGTGGCGGGTAGGAAGGGTGACCTGGTCTCGAACTGAGAGGACACATTTAAACCCGCATTTGACTGCCCAGAGCATTGATGATCAGTGTCCATGTGTGAGGTTTTTTTTTTGCTTTTTTACTTGGCA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TAAACCCGCATTTGACTGCC

59.11

CACTAGTGCTCAGTCCCAGG

59.47

170

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TAAACCCGCATTTGACTGCC

59.11

CTGTTCACAGCAGCGACACT

60.88

147

PCR template sequence:

CTCGAACTGAGAGGACACATTTAAACCCGCATTTGACTGCCCAGAGCATTGATGATCAGTGTCCATGTGTGAGGTTTTTTTTTTGCTTTTTTACTTGGCAGTAAGTGCAGCTGTCATGCCTCCGTGCCCTAAAGTGGTGCTTGCTTGCAGTGTCGCTGCTGTGAACAGGGGCCTGGGACTGAGCACTAGTGTATTTTGTA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

21

Interacting miRNAs (#binding sites)

mmu-miR-709(2), mmu-miR-490-3p(2), mmu-miR-338-3p(2), mmu-miR-9-5p(1), mmu-miR-134-5p(1), mmu-miR-31-5p(1), mmu-miR-205-5p(1), mmu-miR-29b-3p(1), mmu-miR-335-5p(1), mmu-miR-29c-3p(1), mmu-miR-339-5p(1), mmu-miR-214-3p(1), mmu-miR-22-3p(1), mmu-miR-212-3p(1), mmu-miR-297a-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-377-3p(1), mmu-miR-1198-5p(1), mmu-miR-3065-5p(1), mmu-miR-540-3p(1), mmu-miR-296-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TTTTTACTTGGCAGTAAGTGCAGCTG

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.707003695

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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