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mmu_ciDis3_884
Basic information of circular RNA
PanCircBase ID:
mmu_ciDis3_884
mm39_Chr. Position ID:
chr14|99325313|99326197|-
Gene name:
Dis3
Transcript:
ENSMUST00000042471.11
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
9
Exon/intron offsets:
0
Genomic length:
884
Exon/intron sizes in nt:
884
Flanking exon/introns:
chr14:99325286-99326197
Splice length:
884
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTCAGTCAGAGCACTCTGCAGTGACGTTCACATTGCTAGCTTATCCCACAGTGTGTGTTGCTTTGTTGTTAAATTGGTGATTGAACCTAGGGCCTTATGCAAGTCAGTCAGACACTCAACCACTGGAGTAAATCCTTGGCCCTATTTTACATCTTGGTTTTTCTTTTCAGTTGGGTTTTTTACTATGTTAGCCAGTTTGCCCTGGATCTTTGTCCTGCCTTAGATTCCTGCATATCTAGGAACCAGACCACGAAGTAGTTTAATTTCTACATATGTCTTTAAATAATGTAGAAACCATGGCTGCTGCTTCACAGTTAGTTAGTTCCAGCATTATTAGCTGTTTACTGTTCTAAAGGTAGTGGAGCCTGGGAGTATGGAGACTAAAAATGAGGCAGACTAAAGTCCTCTGCAATAGCCAACTTTATTCAGAGCATCAGACAATTTATACTGAGGGTTGAATATAGGTAATCAAGTAAGGGGTACAATTACATGGACAAGTCACATGTGTCAAAAACATGTTTTTTCCAAAGAGGCATTTAAGCAAATAACAATAGCCAGCTGTGATGAATAATCTAAACTCACTCCTATCTCTACAACTAGGAGCACGGAAGCATAGTCCAAGAGCAGTTTCATGTTTCTAAAGAAATGATGTTTCTAAAGACTCTTGTTTTCCTGGAGTTTTCTCGTCAGCACTCAGGATTCTCTTTTTAAGGTGTTACAGGAATCATTTGCTCTTTTCATTGAAAGATGATGTCTCCAGGCTGTAGAATTAGGAGCTAGCATAGAACGAGTGTCTCTACAACATGGAAAGCCGACTTATTTTTATGGAAATATTGGTTTCCCAAAGAGCAGTGTTGCGATTGTATCCTCCTGGTTTTCTGTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTTTCCCAAAGAGCAGTGT
60.06
GGCCCTAGGTTCAATCACCA
59.38
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTTTCCCAAAGAGCAGTGT
60.06
AGGCCCTAGGTTCAATCACC
59.08
145
PCR template sequence:
GAACGAGTGTCTCTACAACATGGAAAGCCGACTTATTTTTATGGAAATATTGGTTTCCCAAAGAGCAGTGTTGCGATTGTATCCTCCTGGTTTTCTGTTGGTCAGTCAGAGCACTCTGCAGTGACGTTCACATTGCTAGCTTATCCCACAGTGTGTGTTGCTTTGTTGTTAAATTGGTGATTGAACCTAGGGCCTTATGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-let-7e-5p(1), mmu-miR-30e-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-384-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-673-5p(1), mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGTTTTCTGTTGGTCAGTCAGAGCA
siRNA sets
ACTGACCAACAGAAAACCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIDIS3_884_ORF_3
0.8157
-0.198691011
0.021411849
105
ORF sequence:
ATGGAAAGCCGACTTATTTTTATGGAAATATTGGTTTCCCAAAGAGCAGTGTTGCGATTGTATCCTCCTGGTTTTCTGTTGGTCAGTCAGAGCACTCTGCAGTGA
Peptide sequence:
MESRLIFMEILVSQRAVLRLYPPGFLLVSQSTLQ
IRESfinder result
IRESfinder score
0.8538089
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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