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mmu_ciDele1_627
Basic information of circular RNA
PanCircBase ID:
mmu_ciDele1_627
mm39_Chr. Position ID:
chr18|38390453|38391080|+
Gene name:
Dele1
Transcript:
ENSMUST00000025314.7
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
8
Exon/intron offsets:
0
Genomic length:
627
Exon/intron sizes in nt:
627
Flanking exon/introns:
chr18:38390453-38391080
Splice length:
627
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTACCGTTTCATCCCCAGACCCAGTGAGCTCAAAAACTGAGCAGTAAGCTGAAGGGGATCATAGTTCCACTTCTCTGCCTGGCTCTGGGTCTGTTGGAGCCCAGCTGTTAGACCAAAGTAATATCTTCCTAACTTACAGAAACATACTCAGAACTCAGGGGGTGCAGACAGTTTGCCTTCCCAGAGCCTAGATTTGTGCCCCGATTAAGAAATTAATTTTAAGACAGAAAGATAGGTATTCAAGGGTGTCATAATTGACCATGGTAACACATACCTATAATTCCATCACTCTGGAGGCTGAGGCAGGAGGATCTCCACACATTTGAGGCCAATCGGAGCTCTACAGATAGTAACAGATCTGCTGATAAAGAACTCTTGATATAGAGCTCTGTATCAAGACCTCACCTCAAAAAGACAACAGATCAGAAAAGCCCGCAAAAGTCCTAGTGTTCTTTAGAAGGTAGGATATACTGGGGTTTTACTTTCGTCTTTTGGATCTTTGTTTTTAAATTTTGACTTACCAGCATTTACCTGACATAATGATTATGCATTTATTTGAATGATTTTTAAAATTTCAAAGAGGCAAGTTACCTGCAATCAATCTGGTTATCTCTCCCATTGCCCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAAAGAGGCAAGTTACCTGCA
60.16
CAGAGCCAGGCAGAGAAGTG
60.39
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAAAGAGGCAAGTTACCTGC
58.5
CAGAGCCAGGCAGAGAAGTG
60.39
140
PCR template sequence:
TTACCTGACATAATGATTATGCATTTATTTGAATGATTTTTAAAATTTCAAAGAGGCAAGTTACCTGCAATCAATCTGGTTATCTCTCCCATTGCCCCAGGTACCGTTTCATCCCCAGACCCAGTGAGCTCAAAAACTGAGCAGTAAGCTGAAGGGGATCATAGTTCCACTTCTCTGCCTGGCTCTGGGTCTGTTGGAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-185-5p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCCATTGCCCCAGGTACCGTTTCATC
siRNA sets
ATGAAACGGTACCTGGGGC, GATGAAACGGTACCTGGGG, AACGGTACCTGGGGCAATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIDELE1_627_ORF_1
0.8976
-0.196271206
0.025924448
108
ORF sequence:
ATGATTTTTAAAATTTCAAAGAGGCAAGTTACCTGCAATCAATCTGGTTATCTCTCCCATTGCCCCAGGTACCGTTTCATCCCCAGACCCAGTGAGCTCAAAAACTGA
Peptide sequence:
MIFKISKRQVTCNQSGYLSHCPRYRFIPRPSELKN
IRESfinder result
IRESfinder score
0.898699348
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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