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mmu_ciDdah2_787
Basic information of circular RNA
PanCircBase ID:
mmu_ciDdah2_787
mm39_Chr. Position ID:
chr17|35278248|35279035|+
Gene name:
Ddah2
Transcript:
ENSMUST00000007255.13
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
1
Exon/intron offsets:
0
Genomic length:
787
Exon/intron sizes in nt:
787
Flanking exon/introns:
chr17:35278248-35279078
Splice length:
787
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTCTGAAGAGGGGGCTGTCTCCGAAACGGTATGGGGAGACTGGAGTTTGGGGGTGCTGCATACGTCCCTGCACCGGACGGAGTGTGCGAATAACCTGCACTTGGGCGAAGAGCTGGGCGGGGTAGACTTTAGAAGATGGACAGGGGTACGGAGTTCTGAAGCCTGGAGTGGCTCCACGCCGCGCCCCTCCCCCGTTTCTCCGCCCTCCAGATTTCGTTTAAGACCTCTCCCAGTCCTCCGGGACTAAGTCTGGTTTGTACTGGGTCGCTCGAAGGGCAGCGTGACGACCCGGGGTGGAGAGAGAGGATGCTTAACTCCTTAGATTCTAGTTCCTCGGGTTTGCGCCCGTTTCCCTTTCTCCTTCGAGCATACGGGACTTCACACCCCAGGTCCTGATTCACCACCCAGTTTTCTTTTCCAGTCCTATAACCTCTGCGGGTCCCTGGCAGGACTTTGGGTCCCTCCTGCTGCGAGCAGCTGAGGGTTAAGGGGGCGGGGCCGCCGCATTTTTGGGGAGTGAGCGCATCCTAGCGGCTGCCAGGAGGGGCGCGGGGCAGGGACCTAGCGGAGCCCCGAGCAGGGGCAACAGGTGTTTGGGAGATTAAGGATCCAAGTCTTGTTCCCTTGGGCGCCTCTTAAAGCAGATCCCCGGTGAAAGGAGGCTGAGCGGGGTCCACAGAGCCAGAAGGTTTGGAGGGGGTGGGGCAGAGTGGGAGGGGCGCCTGGAGATGGCAGGAGGAAGACCTAGGAGCTTTTAACACTCACCCCTTCTCTTCTTTGCAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGATGGCAGGAGGAAGA
59.96
TTATTCGCACACTCCGTCCG
60.46
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGATGGCAGGAGGAAGA
59.96
AGGTTATTCGCACACTCCGT
59.39
160
PCR template sequence:
AGAAGGTTTGGAGGGGGTGGGGCAGAGTGGGAGGGGCGCCTGGAGATGGCAGGAGGAAGACCTAGGAGCTTTTAACACTCACCCCTTCTCTTCTTTGCATGTGAGTCTGAAGAGGGGGCTGTCTCCGAAACGGTATGGGGAGACTGGAGTTTGGGGGTGCTGCATACGTCCCTGCACCGGACGGAGTGTGCGAATAACCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-150-5p(1), mmu-miR-298-5p(1), mmu-miR-320-3p(1), mmu-miR-149-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
CTCTTCTTTGCATGTGAGTCTGAAGA
siRNA sets
ACTCACATGCAAAGAAGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.569613791
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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