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mmu_ciDaam1_317
Basic information of circular RNA
PanCircBase ID:
mmu_ciDaam1_317
mm39_Chr. Position ID:
chr12|71998906|71999223|+
Gene name:
Daam1
Transcript:
ENSMUST00000223272.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
14
Exon/intron offsets:
0
Genomic length:
317
Exon/intron sizes in nt:
317
Flanking exon/introns:
chr12:71998906-71999225
Splice length:
317
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGCCATTTGTTCCCATTTTCCCATTAGTGGATGAGTGGGTGGCACCTGTAGCTCTGTGTTCTCAGAGGGCAACCGTCCCTGCTGCTGGGTAACTTGACCATCTAGTGTGGACTCAAGGTTCTCACCAGCTCCTATGTTAGTTGACAATGTCTCTGTAATAATTATACATTGCTTCAAACCAGCTCACTGGAACATCCTGTAGAATTATAAATTTGAGATAATGGTACTTAAAAAAAAAAAAACCAACAATCCACCCTACTTACTCTTTTTTCTGTATGACTGTCAATCATGGAACTTGTTAACTTGCTGTTTCT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAACAATCCACCCTACTT
56.33
TCAAGTTACCCAGCAGCAGG
59.96
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACCAACAATCCACCCTACT
56.33
TCAAGTTACCCAGCAGCAGG
59.96
174
PCR template sequence:
GAGATAATGGTACTTAAAAAAAAAAAAACCAACAATCCACCCTACTTACTCTTTTTTCTGTATGACTGTCAATCATGGAACTTGTTAACTTGCTGTTTCTGTAAGCCATTTGTTCCCATTTTCCCATTAGTGGATGAGTGGGTGGCACCTGTAGCTCTGTGTTCTCAGAGGGCAACCGTCCCTGCTGCTGGGTAACTTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-503-5p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTTGCTGTTTCTGTAAGCCATTTGT
siRNA sets
CAAATGGCTTACAGAAACA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIDAAM1_317_ORF_1
0.506
-0.278609792
0.016427943
189
ORF sequence:
ATGGTACTTAAAAAAAAAAAAACCAACAATCCACCCTACTTACTCTTTTTTCTGTATGACTGTCAATCATGGAACTTGTTAACTTGCTGTTTCTGTAAGCCATTTGTTCCCATTTTCCCATTAGTGGATGAGTGGGTGGCACCTGTAGCTCTGTGTTCTCAGAGGGCAACCGTCCCTGCTGCTGGGTAA
Peptide sequence:
MVLKKKKTNNPPYLLFFLYDCQSWNLLTCCFCKPFVPIFPLVDEWVAPVALCSQRATVPAAG
IRESfinder result
IRESfinder score
0.873830245
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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