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mmu_ciCul2_523
Basic information of circular RNA
PanCircBase ID:
mmu_ciCul2_523
mm39_Chr. Position ID:
chr18|3425571|3426094|+
Gene name:
Cul2
Transcript:
ENSMUST00000025073.12
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
12
Exon/intron offsets:
0
Genomic length:
523
Exon/intron sizes in nt:
523
Flanking exon/introns:
chr18:3425571-3426094
Splice length:
523
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGTTTAGTTGCTTGAAGGTAATCTCATGTGAAGTTGTAAAGGGAAGTTACTAGAATGGCAAATTAATTTGTGTACTCATTTTCAGAATATGAAAATTGTCCGCATTATATATATATTTAAATTTACGTTCAGAATGCATTAAACAATGTTCCTTTTTTTAACCAAAGGAGAAAATATTTCATAAAGATGATAATCCTGTTAAAAAGGCATGCATACTATTCATTATCTGATTAGGTTTTTTTCTTAGTTCTAGTGTCTTATGTTTTTAAAACTTAGCTGATCACGCATGTTTTACATTCATAATATTAAAGTATAACTTTAGGATGTAACAGCTTTCAACATCTGTTGAGCCACTTTTGCTTTGCTTTTGTCCCATCGATGGTAACCGTTTACCGGCTTGTTTTCATGTTTATGCTCTTACATCTGTTCTAAGTGACAAACCTATGAGGCTTTGTGTTGCCGCCTTTGAAGTGTCTGTCCAAAGGGCCTTTGTAAGATGTATTATTACTTTCTCTTTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTATGAGGCTTTGTGTTGC
58.84
TGCCATTCTAGTAACTTCCCT
56.05
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTATGAGGCTTTGTGTTGC
58.84
TGCCATTCTAGTAACTTCCCTT
56.8
144
PCR template sequence:
CATCTGTTCTAAGTGACAAACCTATGAGGCTTTGTGTTGCCGCCTTTGAAGTGTCTGTCCAAAGGGCCTTTGTAAGATGTATTATTACTTTCTCTTTGAGGTAAGTTTAGTTGCTTGAAGGTAATCTCATGTGAAGTTGTAAAGGGAAGTTACTAGAATGGCAAATTAATTTGTGTACTCATTTTCAGAATATGAAAATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-369-3p(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTTCTCTTTGAGGTAAGTTTAGTTG
siRNA sets
TAAACTTACCTCAAAGAGA, ACTAAACTTACCTCAAAGA, CTAAACTTACCTCAAAGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CICUL2_523_ORF_1
0.7462
-0.459619159
0.005626934
81
ORF sequence:
ATGAGGCTTTGTGTTGCCGCCTTTGAAGTGTCTGTCCAAAGGGCCTTTGTAAGATGTATTATTACTTTCTCTTTGAGGTAA
Peptide sequence:
MRLCVAAFEVSVQRAFVRCIITFSLR
IRESfinder result
IRESfinder score
0.92763274
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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