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mmu_ciCluh_613
Basic information of circular RNA
PanCircBase ID:
mmu_ciCluh_613
mm39_Chr. Position ID:
chr11|74551582|74552195|+
Gene name:
Cluh
Transcript:
ENSMUST00000117818.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
9
Exon/intron offsets:
0
Genomic length:
613
Exon/intron sizes in nt:
613
Flanking exon/introns:
chr11:74551582-74552222
Splice length:
613
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGACTACAGCCTTAAGCTTCCCTGTAGCTGGGCTTATGGGTAAAGAAAACTAGAGGCTGTCACTAGCTTACCTTTGTTGGCAACACTGGAGATGGACTTGCAGGTTATGTCAGCCTCCTACCACCTAAGGTCTTTGCTATGGCTTCACTGAGATTCTTGAGACCATAGAATGGGGAGTAACCTATCTTTCTTGGCCATCTTCATGTCCTGCCATGATCACATGGTGCTCTTACTGAGCCACAGGTGCAGTAGCACACTGATAGAATGTGTGTGCACATGTGTAGTGGGTATCGGGCAGGGCAATCAGTGGGAGACAGGTCTCTCCTCTTACTGTAGGTAGGTCCAGGGATTGAAGTGATGTAGTCAGACTTGGTGGCAAGGACCCTTACCTGCTGAACCATATCATTTGGCCCTGCATACTCCCTTTTGTTCCTGATTGTTTTTTTTTTTTTTTTCAGTAGAATTGTTGAGGTCTCAAAATGATTTTAAGAAATGGTGCTGTTCCCATTTCTAAATATGAAGAAACTGAGGCTAGGGTTCCCTGGTGTCCAGGGCCTGGCTGCCTTTACCATCTAGGTGACCTGGGCCAGAATAGGATGTACCTTGGTGCTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGGCTGCCTTTACCATCT
59.74
GTCCATCTCCAGTGTTGCCA
59.96
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGGCTGCCTTTACCATCT
59.74
AGTCCATCTCCAGTGTTGCC
59.67
157
PCR template sequence:
AAATATGAAGAAACTGAGGCTAGGGTTCCCTGGTGTCCAGGGCCTGGCTGCCTTTACCATCTAGGTGACCTGGGCCAGAATAGGATGTACCTTGGTGCTCGTGACTACAGCCTTAAGCTTCCCTGTAGCTGGGCTTATGGGTAAAGAAAACTAGAGGCTGTCACTAGCTTACCTTTGTTGGCAACACTGGAGATGGACTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-29b-3p(2), mmu-miR-29a-3p(2), mmu-miR-29c-3p(2), mmu-miR-199a-5p(1), mmu-miR-216a-5p(1), mmu-miR-25-3p(1), mmu-miR-24-3p(1), mmu-miR-362-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TACCTTGGTGCTCGTGACTACAGCCT
siRNA sets
TAGTCACGAGCACCAAGGT, GTAGTCACGAGCACCAAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CICLUH_613_ORF_1
0.9105
-0.144432309
0.052561058
177
ORF sequence:
ATGATTTTAAGAAATGGTGCTGTTCCCATTTCTAAATATGAAGAAACTGAGGCTAGGGTTCCCTGGTGTCCAGGGCCTGGCTGCCTTTACCATCTAGGTGACCTGGGCCAGAATAGGATGTACCTTGGTGCTCGTGACTACAGCCTTAAGCTTCCCTGTAGCTGGGCTTATGGGTAA
Peptide sequence:
MILRNGAVPISKYEETEARVPWCPGPGCLYHLGDLGQNRMYLGARDYSLKLPCSWAYG
IRESfinder result
IRESfinder score
0.765924233
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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