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mmu_ciCep192_423
Basic information of circular RNA
PanCircBase ID:
mmu_ciCep192_423
mm39_Chr. Position ID:
chr18|68011146|68011569|+
Gene name:
Cep192
Transcript:
ENSMUST00000025425.7
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
42
Exon/intron offsets:
0
Genomic length:
423
Exon/intron sizes in nt:
423
Flanking exon/introns:
chr18:68011146-68011609
Splice length:
423
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGTATTCTCCTGGATACAATAAGCAAGTATGTGTCTATTAAAACCCTAAATGAAAAAACATCTCAAAATACACAGTTATCCCCTGATACGGTTTCAGCTTTCGCTGATATGTGGCGTGGCCTCACTCATGCTTTAAGATTCTTCTCAGACTTTATTAGTTGCTGTCTTTGCAGTCTCTGTTGTCTAAACTCCACATAAACCATGGTTGACCTAACTATGTAAGTAACTGTCTATGTGTATGTATGTGTATGGGCATTTGCATTGGGGGCAGCAAAACTAGACAGTATACAGAACAGAGTTTGCAGGATTTAAAAATAATCATGTCTTTACAGTTTTTTGTTTGTTTGTTTGTTTTTGTTTTAATAATGAAATTCTCATGAGGAAATGTCTTTGTTCTTTCTGGAGTATTCTCCTTTTCTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTCATGAGGAAATGTCTTTGTTCT
58.46
CCGTATCAGGGGATAACTGTGT
59.3
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTCATGAGGAAATGTCTTTGTTCT
58.46
ACCGTATCAGGGGATAACTGTG
59.3
143
PCR template sequence:
ATGTCTTTACAGTTTTTTGTTTGTTTGTTTGTTTTTGTTTTAATAATGAAATTCTCATGAGGAAATGTCTTTGTTCTTTCTGGAGTATTCTCCTTTTCTCGTGAGTATTCTCCTGGATACAATAAGCAAGTATGTGTCTATTAAAACCCTAAATGAAAAAACATCTCAAAATACACAGTTATCCCCTGATACGGTTTCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-429-3p(1), mmu-miR-19b-3p(1), mmu-miR-466d-3p(1), mmu-miR-466b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCTCCTTTTCTCGTGAGTATTCTCC
siRNA sets
AATACTCACGAGAAAAGGA, GAATACTCACGAGAAAAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CICEP192_423_ORF_1
0.4251
-0.244061935
0.022754392
237
ORF sequence:
ATGTCTTTGTTCTTTCTGGAGTATTCTCCTTTTCTCGTGAGTATTCTCCTGGATACAATAAGCAAGTATGTGTCTATTAAAACCCTAAATGAAAAAACATCTCAAAATACACAGTTATCCCCTGATACGGTTTCAGCTTTCGCTGATATGTGGCGTGGCCTCACTCATGCTTTAAGATTCTTCTCAGACTTTATTAGTTGCTGTCTTTGCAGTCTCTGTTGTCTAAACTCCACATAA
Peptide sequence:
MSLFFLEYSPFLVSILLDTISKYVSIKTLNEKTSQNTQLSPDTVSAFADMWRGLTHALRFFSDFISCCLCSLCCLNST
IRESfinder result
IRESfinder score
0.917541141
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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