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mmu_ciCct8_636
Basic information of circular RNA
PanCircBase ID:
mmu_ciCct8_636
mm39_Chr. Position ID:
chr16|87287549|87288185|-
Gene name:
Cct8
Transcript:
ENSMUST00000175977.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
4
Exon/intron offsets:
0
Genomic length:
636
Exon/intron sizes in nt:
636
Flanking exon/introns:
chr16:87287270-87288185
Splice length:
636
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATTCATCTTGTACTTTAATTATATTTGAGTACTTGTGTGGTTTTGCTTAAAGCTTAAAATTCAGGTCTAGATGGGTAACAGCACTCGTGGGCAAGCACAGGGACCCGAGTAGTACCCGGTTATACCTGTGATCCCACATGGAGGAGTGAAGCCAGGAGTATCACTGGAACCTGCTCACCAGATTGAGCAGGACACCTGAGAGCCACTGTGCATGTGCGTGCCTGGACAGTCACATGGGCTTACGGTGTACATATATATTGTACCTATTCCAATATGTGTACTTTTACCATATACAAGTAAATGATTTTAAAATAAACTTTAAATCTACTAGTAATAACATTGAGTAGTTTCCATTTTAGGCATTGGCCATATGCTTCGTGTGAATTTGTCCTCAAAAGATTCCTATTGCTAAGTGTTCTTAAAGCCAAGGTCCTCTGAGTGACAAGTGTTAGAGTAAGGGCTGCTATTTGTGTTGTCATCAGAGCCGTGTAGTCCATAGCTATTGGCTCTGCATGCATTTCTGCAAGCACTTGCCATCTTATGTGAAAATCCCCACATTTGTTGGATTACCAGAGTAAGTCATGTCAAAAGTATGTCTACTAAAACGGTTATTTTTATATCATGGTTTAGGTAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCACATTTGTTGGATTACCA
58.83
CCACGAGTGCTGTTACCCAT
60.04
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCCACATTTGTTGGATTACCA
60.18
CCACGAGTGCTGTTACCCAT
60.04
177
PCR template sequence:
CATCTTATGTGAAAATCCCCACATTTGTTGGATTACCAGAGTAAGTCATGTCAAAAGTATGTCTACTAAAACGGTTATTTTTATATCATGGTTTAGGTAAGTATTCATCTTGTACTTTAATTATATTTGAGTACTTGTGTGGTTTTGCTTAAAGCTTAAAATTCAGGTCTAGATGGGTAACAGCACTCGTGGGCAAGCAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
ATGGTTTAGGTAAGTATTCATCTTGT
siRNA sets
ATGAATACTTACCTAAACC, ACAAGATGAATACTTACCT, TGAATACTTACCTAAACCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CICCT8_636_ORF_3
0.6846
-0.641287559
0.002831964
105
ORF sequence:
ATGTCAAAAGTATGTCTACTAAAACGGTTATTTTTATATCATGGTTTAGGTAAGTATTCATCTTGTACTTTAATTATATTTGAGTACTTGTGTGGTTTTGCTTAA
Peptide sequence:
MSKVCLLKRLFLYHGLGKYSSCTLIIFEYLCGFA
IRESfinder result
IRESfinder score
0.773743995
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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