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mmu_ciCcdc186_858
Basic information of circular RNA
PanCircBase ID:
mmu_ciCcdc186_858
mm39_Chr. Position ID:
chr19|56779514|56780372|-
Gene name:
Ccdc186
Transcript:
ENSMUST00000118592.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
15
Exon/intron offsets:
0
Genomic length:
858
Exon/intron sizes in nt:
858
Flanking exon/introns:
chr19:56779478-56780372
Splice length:
858
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATTTGTGTTTCTTATTTATTTGCCACTCAGTAACCAGGGCAGGATGGCGCCTCTGCCATAGTCTCACCTAGCGTCAGGCTGGTTTTCTTTTTTCTTTTTTTAAAATTTATTTTTTATTTCTATGAATACACTGTCACTGTCTTCAGACACACCAGAAGAGGACGTCAGATCCCATTACAGATGGTTGTGAGCCATCACGTGGCTGCTGAGGATTGAACTCAGGACCTCTGGAAGAGCAGTCAGTTCTCTTAACCGCTGAGCCATCTCTGCAGCCCCAAGCTGTTTTACTAGTACAGGGTGTCTTGGTACTCAGAGATAAAGTGACGTAGTTACTGGGCAGAAACTGAGGCCGATTGTTTGCTTCACAGTGGATGCCGTTTGCAGAATGAGTATACCCAGTTACAACTTCAGTATTACAACATTTAAGTATATTTTGTGGCTTTTTTTTAGCTGTTGTCTCTGACCCAACGTTTCTGTCTTTGCTATGTATGCATGGACTTGATAACTAGGAATAGATTTTAAAAATAGTTTTAAACCAAAGTAATCATTATGCACGAAAACTTGCTGTAGCTGCAGTAAAAACATTATAACGTGGGGCAGGCCGTGGTACCGCACACATTTAGTCCTCACAGAGGCAAGACGCCTATGAGTTTGAAGCCAACCTGGTCTGTGTACAGAGTGACTTCCAGGACTACATAGAGAGAGCCTGTTTCAAAAAAGAATTATAACGGCAATGGGCAGGCCTATTTCGCTAAAACCTCCATGTTTAGACTATGTCAGTACCAAGAATAATCACTCAGCTTCATAGTTTAGACAAAAGTGGTTCAAAAGATATTTACTTAGTACTTCATTTACT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTCCATGTTTAGACTATGTCAGT
59.28
CTGCCCTGGTTACTGAGTGG
60.04
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTCCATGTTTAGACTATGTCAGT
59.28
CCTGCCCTGGTTACTGAGTG
60.04
145
PCR template sequence:
ACCTCCATGTTTAGACTATGTCAGTACCAAGAATAATCACTCAGCTTCATAGTTTAGACAAAAGTGGTTCAAAAGATATTTACTTAGTACTTCATTTACTGTATTTGTGTTTCTTATTTATTTGCCACTCAGTAACCAGGGCAGGATGGCGCCTCTGCCATAGTCTCACCTAGCGTCAGGCTGGTTTTCTTTTTTCTTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-155-5p(1), mmu-miR-134-5p(1), mmu-miR-146a-5p(1), mmu-miR-298-5p(1), mmu-miR-127-5p(1), mmu-miR-382-5p(1), mmu-miR-466d-3p(1), mmu-miR-384-3p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-3470b(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TACTTCATTTACTGTATTTGTGTTTC
siRNA sets
AAATACAGTAAATGAAGTA, ACACAAATACAGTAAATGA, AAACACAAATACAGTAAAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CICCDC186_858_ORF_1
0.6168
-0.448833954
0.007802086
156
ORF sequence:
ATGTCAGTACCAAGAATAATCACTCAGCTTCATAGTTTAGACAAAAGTGGTTCAAAAGATATTTACTTAGTACTTCATTTACTGTATTTGTGTTTCTTATTTATTTGCCACTCAGTAACCAGGGCAGGATGGCGCCTCTGCCATAGTCTCACCTAG
Peptide sequence:
MSVPRIITQLHSLDKSGSKDIYLVLHLLYLCFLFICHSVTRAGWRLCHSLT
IRESfinder result
IRESfinder score
0.872697459
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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