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mmu_ciBtrc_4496

Basic information of circular RNA
PanCircBase ID:

mmu_ciBtrc_4496

mm39_Chr. Position ID:

chr19|45491602|45496098|+

Gene name:

Btrc

Transcript:

ENSMUST00000223764.2

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

2

Exon/intron offsets:

0

Genomic length:

4496

Exon/intron sizes in nt:

4496

Flanking exon/introns:

chr19:45491602-45496122

Splice length:

4496

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTATGTGCCCTTCCTGTAACGTCTTAGTTCATTTGCTACAGCAGTAGTGGAATTGGATCTTCAACCTTGCTCTCCCAGCCCTGCTTCTCCGTAACCTCAGTAGCCTCAGATTCACTGTGCAGCCAGGACAAGCCTCAAACTCAGCAGTCTTCTGTCTCAGCATTGAACGTGCTGGAAGGCCAAGCATGGTGGTGCGCGCCTTTAGTCTCAGCACTTGGGAGGCAGAGGTAGGTAGATCTCTGTGAGTTCGAGTCCAGGACAGCCAGGACTCTGTTACACACAGAAACTCTGTCTCTGGGGTGGGGTGGGGTGGGGTGGGGTGGGAGATAAAGGAAACTAACTGCTGGGACTACAAGTGTCTCACTCCTCGATACATTTCTCTTTTTAACATTTTTGTTTGGAGGCTGGAGAGCTGGCTCAGTAGTTAAGAGCACTGGCTGTTCTAGAGGACCTGAGTTCAATTCCCAGCACCCACATGGTGGCTCACAGATGTCTTTAAGTCCAGTTTCAGAGGATCCAGCAACCTTATACAGACATGCATGCAAGCAAAACACCAGCGCACAGGAGATAAAAATAAATAAATTTAAAATTTATGGTTTGGTTTGATAGTTACACACCAAGTCATTAATTTTACTTTCATCAAAATAAAAGAAAAACCAAACTGCAGAATTAAATTCCATTTTACTTTGTCTCCTTGCCCCTCTGCTCTGCCTTTTTGTTTAACCCATGTGCAGTTCCAAAGCTAGTTATATTTTTTGTCCGTGTTACCTGACTCGAGGCCCGACTTGTATTCTACGTTATTATTGTCAATTCCTTTCATGGACTTGGATTTAAATGTGAATTTTAGCAACAAGAAATTGTATGTTTGTTTTGTTGTTGTTGTTGTTGTTGAGGAGACATTCTCTCTCTAGCTCAAGCTGGCTAAAACACCGAGGTCCTCGTGCGGTGGCTTCTACCACATCCTGTAACAGTGCTTACATTGTAGGAATTAATTTTTGGTTTTTTCGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTAGAACTCACTTTGTAGACCAGGCTGACCTCGAACTCAGAGATCCGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCGTGCACCACCACCACCTGGCTAGGAATTAATTTTAAAAACAACATTTTGTAAATCTTTTGGAAAGAAGGTTCAGTTCTTGCTGTGTGTATCATCCCTCCGCTCCCTCCTGCCATGCATGCGCACACGAGAGACACTTGAGATGCCCTCGTTTTCTGTGGATCAAGCCAGGGTCTTGTTTGTAGAGAGCAAACACACTACTATTGACCTGTCCCCCCATCCCTGATGCAGGAGCTGTATGGGGATAGGAAGTAAACCCCCGAACCTGTGCAAACTAGATAAATACTCCATCACTAAGTAAACACCTCCAGAGTGTTTCCCTCTCTTCTTTCATGGTGATCCAAAACCCCGCAAAGCCTGAGAAACAGGATGTGTTTATGTAGGCTCCACTTTCATTAGGCTGTGAATACTTATAGTCAGAGTCAATAAATGCCGGGTCTTGTTTGAGGGTATTTTTCTGTTTTGACTGGTTTTGATGGTCATATGAAATTTAGAAAATACAAATGTGTGTGTGTGTGTATATATATATATATGTATGTAAATATATTTTTATATATGAATGATAGCTCAGCCAGTAAAATATTCACCATGCAAGCTAAGTACCCGAGTTTGATCCTCAGCGTCTATACAAGAGAGCTGAAAGAGGGGGTACATTTGTATTCCCAGACCTAGGGAGGCAAATGTAAGCAGATTCATGGGGCTCAGTGGCCTCCATTCACATGTATGTGAACACACACATACACACAAAATATAAAACACAATTGCCCGGAATTCTACCACAGAGACCTCTTTGTTTTTCTTATAAAGTTCTGATCATGTGTTTCTTTCCCTGTAGATCTGGAGTTTGTTGGGGTTTTTTTTTTTTTCTTTTTAATTTTTCTTAATATAGTCTCCTAGTATATTTTTATTAAATATGTTAAAATTCTATTTTTAAATAACTTTAGTCTTATACAGTACGTTTTGCAGTAGCGAACACATTATTTAACCCCTTTGTTAATCGTTTTCTGTATAAATTATACCATGGTAGACATTCTTATGCATTAATCTCCTAGACAGTCTCAGAAGAAAAACACCCAAAGTAGAATTGCTGTATTAAAGAATATGGGGAGTTTCAGTGGGTGTAATTTTGTTTTGTTTGTGTTTATTTTGGCTTGATGGTTTTGTTTGTTGTTTTTGGGTGTTTTGGGTTTGGTGTTGGAAATTGAACCACTAACCACTAGCCACTAGCCACTAACACCACCACCACCACCACCCCACCCCACCCCCGCTGCCCCGAAGCAGGAGCCTTTTAAAAGGATTCACTTCCCTTTGAGGTAGGGTCTTACTGTGTTCCGTGACCTGGCCTCAGACTTTACTATCCTCCTGGGATTGCAGCTGTGCGCGCACCCCGCTCACCTTGTCTGTAAAGGGCTTTTGGCCCATGCTGCCACACTGTCCTCCATGCACACTGAACAAACCGAGCCACTTCACAGCTAGCTGTGGAAGTGAGTGCTGCCTCTCTGAATCCTTGGCAGCTATTTCTTAAAGTCTTGTTTTTCTGAACTTAAAATAAATTACCCTTTAAAATTAAATTACCCTTTACAATTAGTTATTTGTATATTATGAATTACCACTTTTAAACAGAATTTTGCCTATATTCTTTGCTTAAATAGAATAGGTTTTGTCCTCTTGTAAAATACTGGATTTTGTGTAGCCATAGGCAAGCAGTTTTCTTTAATGCAATCTGAGTACACCTCTTGCTGTTGTCGTCCCAATCACTGAGACATGCTACTGTCTCAGGCTAGGCACTAAGGCCAGTGCTGATTGGATCAGGGTATTTTGCCAGATACTGGGCTGATCCCTAGTGTGTCTCTGATGGCCAGGCTGTGTTGTATTAAATCGTGTCCAATGCTTAGCAGCCACTTAAGTTTCTCAACCTACACCATCTATCTGTGTTTAGTAAACCAAACTACTTCAAAAATTGCCTGTAATCCATTAATGTAATGGTTTTGTTTGTAGTGTTAGGTGAAATTACTTCCAGGGACTTATTGCTGGGACATTGTAAAAGATCAGGAGCAATTTGATGACAGCCATTAAAAATGTAAGAATGCCTTTTGGAAACTTGTGATGTTGTAGAAAGTTTAAATATAAGAGATTCAGGAAATATCCCATCATTGTATTTTGTATGAAGAAGTGTTTGTTATCTTTAATTGGCTTATAGTCATATTCCTTTAATAAGAACTAGGTGATGGATGCCTCCATCTGCTTCTGCTTAAATATGAACATGTGATGCCAGCCACACAAGATCCATTTCAAGCTTGCTTTCCCCAGATCTGCTGGAAAATTTGACAGATAGCCATACAATTTTTATAATTAGAATAATGTTTAATAAGCATATTTGGATTGTTGCTAATGGCAGCCAAGTGTGCCTCCATATGATTCGGGGCAACATGCAAAGTAAAATGCATGCTGTCGGTCCTTTCCGTTCATGCCTGCAGACAGCAGACTGGAGGAATCCTTCCCCTTGTGAGCCTTGAGAAAGCCTCGGGGATGACTAGTTGTGACTGGCTGACCTGAGAACTTAGGACAGCAATGCCAGAAGTGTTATTAGAGTGGCCATCCTGTTTGACAGGCTCTTCTCCACCGAGGCTTTGGGGCCTATTGGGTGCTAAAGAGCTTTAATTGGGAAGTACGGTATCAGCCTGCCATATTTTTCCTTTGAAACGCTACCCTCATTTACTGGTAAGATTTTTTTATTCCATTTTAGGTCAGTTGTCACTTAGGTTTTATAGCAGTAAAATTTGTTTTGTTTTGTTTTTAGCACATGCAATCAGACTCCTTTGAACCCTAGTATCCCCATGTGTCTCTCCTCTCAGGGTGCTCCTGGGCCCTGTGCTCATCCCTCTCCTGAATGCCCACACGCCACGTCCCAGATCAGTCTGACTTGCTGCCTCTCTCTCCCTAGTACATTTAAAGGATATTTCTTTCGTTGAATAGCTTTGGAACAGCAGCAGTAATAGAACAGAATGAGAAATGGACCTAATATCAGGATTTCGGGGGGTCTGAATCACTGAGTTTGAGATGCCGTCTGTAGAACATCCCCTAGTGTTGAAATCCATATAGATAGATGTGAACTGTTTTCTGGACTCCTTTTTATTTTTAATGTCTTGGGGGGTTTGCTGGTTGTTAGCTTTAACTTCCTTATCTAACAGAAGATGGAAAATACTGTTCATTTCTGTAACCTTATGGAAAAATTATTGACACTCATTTTCCCCAAGTCTCTGTTTGAGCAAAGCTGGACCACGCTGGGGAGAGCGGTGTGCCTTTATCTCAGGCATGTGCTGCTCTTTTAAAGGGCTTTCTGTGAAGAATCGCTCAGCACCCGCTCTGTTCTTTTT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GGGCTTTCTGTGAAGAATCGC

59.87

GAGGTTACGGAGAAGCAGGG

59.82

142

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GCGGTGTGCCTTTATCTCAG

58.99

GTTACGGAGAAGCAGGGCTG

60.74

179

PCR template sequence:

GACCACGCTGGGGAGAGCGGTGTGCCTTTATCTCAGGCATGTGCTGCTCTTTTAAAGGGCTTTCTGTGAAGAATCGCTCAGCACCCGCTCTGTTCTTTTTGTATGTGCCCTTCCTGTAACGTCTTAGTTCATTTGCTACAGCAGTAGTGGAATTGGATCTTCAACCTTGCTCTCCCAGCCCTGCTTCTCCGTAACCTCAG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

34

Interacting miRNAs (#binding sites)

mmu-miR-322-5p(2), mmu-miR-709(2), mmu-miR-466d-3p(2), mmu-miR-669d-5p(2), mmu-miR-3065-5p(2), mmu-miR-466b-3p(2), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-7b-5p(1), mmu-miR-103-3p(1), mmu-miR-15b-5p(1), mmu-miR-298-5p(1), mmu-miR-7a-5p(1), mmu-miR-138-5p(1), mmu-miR-185-5p(1), mmu-miR-339-5p(1), mmu-miR-495-3p(1), mmu-miR-128-3p(1), mmu-miR-127-3p(1), mmu-miR-191-5p(1), mmu-miR-192-5p(1), mmu-miR-215-5p(1), mmu-miR-297a-5p(1), mmu-miR-107-3p(1), mmu-miR-98-5p(1), mmu-miR-499-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-1198-5p(1), mmu-miR-466p-5p(1), mmu-miR-410-3p(1), mmu-miR-539-5p(1), mmu-miR-3087-3p(1), mmu-miR-697(1)

siRNAs for circular RNA silencing
Junction sequence

CTCTGTTCTTTTTGTATGTGCCCTTC

siRNA sets

AAGGGCACATACAAAAAGA, GAAGGGCACATACAAAAAG, ACATACAAAAAGAACAGAG

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.853596268

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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