top of page
mmu_ciBrwd1_1165
Basic information of circular RNA
PanCircBase ID:
mmu_ciBrwd1_1165
mm39_Chr. Position ID:
chr16|95853866|95855031|-
Gene name:
Brwd1
Transcript:
ENSMUST00000099502.9
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
14
Exon/intron offsets:
0
Genomic length:
1165
Exon/intron sizes in nt:
1165
Flanking exon/introns:
chr16:95853859-95855031
Splice length:
1165
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAGGTACTTTAACATGAGAATTCAGGAATTTAAATTGATTTTACAATTTTTTTTTTAATCAGTGTTCTGTCTGTGTATGTATGTGTGTCCTGTATGCGCCTGATGCCTACAAAGGTCAGAAGAGGGCACCAGGTCACTCTGGAACTAGAGATACAGACAGTTGTGAGCGGCCGTGTGGGTTCTGGGAATCAAACCTGCATTGAGAACAAGTGTCTTAACCACAGAGCCTTCTCTTTAGCCCTAGAATTTCTTTTTTAAGGATTTATTTATTTATTTAATATGTATGAATACACTGTCACTTTCTTCAGACACACCAGAAGAGGGCATCAGATCCCATTACAGATGGTTGTAATTCACCGTGTGGTTGCTGGGAATAGAACTCAGGTGTTCCGGAAGAGCAGACAGTGCTGAGCCATCTTTCAAGCCCTTAGCCCAGAAATTTATTTAAATTAATTCATTAATTTTTTTGAGGGTGTGGGGGAATCTGTGTAGCCCTGGATGTCTTAGAATTTGTAGACCAGACTGACCTTGAACTAAAAAGATCCACTTGTCTCTGCCTGCTGTGTGCATGTGGTACCATTTCTGGTTCAGAATTTTAAAACATTTTTTGAAACAGTCAGTCCTGGTTTGGCTTTCACCTTGGTATATATATCGAGGACCTTAAACTTTTTGTTTTGGTTTTTGAGACAGGATCTCTCTGTAGCTTGGAACTCTATAAAATAACCTGCCTGTCTTCCAACTCACTGGGTTGGTTGGTGGCCTTGTCCTTTTTTTTTTTGTTGTTGTTGTTTTTAAATCTGCATTCATTTATTATGATTTTTAGTTCTGTGTCACTGTGCAGAGGTCTGCCCTGAGCTAGAGTGATGGAGCTGACAGCGGTCCTAGGACTTGAACTCTGGCACTCGCACACACTCAAGCACTGAGCCATTTCTCTAGCACCCCCTGAACTTTTGAAATCAGAATTCTTACTCTTTTCCTTGAAACTCAATATAACATAGATGTTATGAATATAATGAATAAACTGTTTTATTTAAGTGAGTCAAATGGGAAATTTTATATGCATATTTAAATCATTGTTGTAGTGTTAAAACACTTATGTACTATGACTTTATAAATTTTCCAATATATAACTTGCAGACTGTCCTTAATATTATGACTTTGTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAATATATAACTTGCAGACTGTCCT
59.93
GCGCATACAGGACACACATAC
59.41
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCAATATATAACTTGCAGACTGTCC
58.78
GCGCATACAGGACACACATAC
59.41
146
PCR template sequence:
TTTAAATCATTGTTGTAGTGTTAAAACACTTATGTACTATGACTTTATAAATTTTCCAATATATAACTTGCAGACTGTCCTTAATATTATGACTTTGTTGGTAGGTACTTTAACATGAGAATTCAGGAATTTAAATTGATTTTACAATTTTTTTTTTAATCAGTGTTCTGTCTGTGTATGTATGTGTGTCCTGTATGCGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-7b-5p(1), mmu-miR-7a-5p(1), mmu-miR-214-3p(1), mmu-miR-128-3p(1), mmu-miR-433-5p(1), mmu-miR-153-3p(1), mmu-miR-340-5p(1), mmu-miR-342-5p(1), mmu-miR-466d-3p(1), mmu-miR-3065-5p(1), mmu-miR-466b-3p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TATGACTTTGTTGGTAGGTACTTTAA
siRNA sets
TTAAAGTACCTACCAACAA, AGTACCTACCAACAAAGTC, GTACCTACCAACAAAGTCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.939828929
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page