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mmu_ciAtp8b1_2683
Basic information of circular RNA
PanCircBase ID:
mmu_ciAtp8b1_2683
mm39_Chr. Position ID:
chr18|64712288|64714971|-
Gene name:
Atp8b1
Transcript:
ENSMUST00000025482.10
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
2683
Exon/intron sizes in nt:
2683
Flanking exon/introns:
chr18:64712277-64714971
Splice length:
2683
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGTTAATGGTAAAAATGTTTTTATAGTAGTCCAGGTTTGCCTAGTCACTGTCGTGCTTGTCAGCTTCAAACAGTGCCTCCCATTCAGGTAACTACTTGCCTGGGTCTATTAATAACCCTCCCTCATTCTATAGACTGTCTTTTTATTAAAATTTAACTACTCTGAAGGTATGACTTGATACAAAATAGAATCATGTCAATTGGAAACTTCCCCTGTGTAGCTGCAAGGTTCTTGCTAAAATATTTTATGAAAATTATGATTGTCACTGAAGTCCTGTTTTGGAAAGTACTGAGTTGACTCTGCGTTGGATGGGTTTAGAGATAGCAAGGATATTTCACCTAAAATGCTTATTTCCTTTAATATATTATAATACATAGACAAGTGGGGTTAGAGAAACGGCTCAGTAGTTAAGAGTTCTTGCTGCTCTTACAGAGGACCATGGTTCATTTCCCAGCATCCACAAGGCACCTCATGACCATTATAGCTCCAGTTCTTGGGAGCATGAGACCCTCTTCTGGAGTCTGCAAGGAGCAGATGTACATGTTGTACACATACATACATTGCAAGCAAAACATTCATGCACATAAAATAAAAATCAGTAAATCTATTAAGAAAGAATACAGACAAGTGTCAGTTAAAAGCAGAGAAAGAACTTATTCAGTGCAGCCACATTGAGAAGAGTAACAAAGAGTGGCCTCCCAAGTCCATCTTCTAGATTCTCCCATGAGGTGAACGATAACCAGAAGACGAGGGTTATGCATAGCTAAGCTGTCCCTGTCGTTATGTTGTCAACCTGCCACTCATTGTGATGGTCTAGTTAGAGCCACAAGGCGATCTTACAAGCTGTTGTAACGGTGTGAAAGCTCTTTCCTATCTTACCTTTGGCTAGGAACATTAAAGTACCCCCATTGTGTGACTTAGAAAGCAGGACATTATTTTCTAGTAGTTAAGGGGATCGCAAGTCCAGAGTCAAGGGGTTGACACGGTAGGGAACTTATGGAAAATCTCAGAATCAGATCCATGCCTGTCCCAACATCTGGTGCTTGCACAGGATTCTAAGCATTCTTTGGCTAGTAAACACATCACTCCAATTCCTGTCCAATCCAGTCTTCTGTCTTCTTCCTGTGTGTCTCTGTATCTAATTCCCACCCCCCACCTTCCCACAAGGCAGTGCTTCTCAACCTCCCTAATACTGCAACACTTTAAAACAGTTCCCCGTATCATGGTATCCCCCAACCATACAATTATTTTTGTTGCTACTTTAATTATTAAATAAAAATAACTTATTAAACTTATTTTATAAGTTATAACTTATAACTTATAAAAATAATTATTAAATAAAAATAACTATAATTATTTTACTGTTATAAGTTCTAATGTGTAAAAGTAACTTTTACTCTCTTGTAGTTATTGCCATCTCTAAGGGTTTCTGCCTCAGTCTGCTAACCTAGGCCTTGTTCTGGAAGCTTCTAACCCCCTTACAGTCTAACCGAGGTCTAGAATGTTTTCAGCTTCTGAGACTTGCTGTCTCACACTTTCTAGCTCTTTCTGAACTCTGGCTGGCTGCTCAACTCAGCTGCTCTGGCTCAAACTCCTCTCCAAGCTGACTGACTGACTCTGGCTTCTCTCTCAGCCTCTGACTTTTTGTTCTGCTTGGCAGCAGTCTGTTCTGATCTTCTGCCTCCTTTCTCATTCACTGCCTTGCTCTGTCTTCACCTGTGTCTAGCTTGTTTTCTCTTCAACCTGTCTCTGGAAAACTCCTGGTAAAACTGCCTACTCTCCTTTCTTCCTTCTTCTATCTCTGCACTACCCTCTTAAGTAGCCTCCTTTCCTCTCTCTTCTTATGAGAGCTGGGCATATCCTATTCTGTTAAATCTTACTCTGATTTGTCACTTTGTCTGCCAGTCAATTAGACATGACGTTCAAACATGCGTGCTTCCTTCTACAAACTAACTTTACCTTCATCGTTTGGGATTAAAGGTGTAAACTAAGGGTGTATCCGTATTCTAGCCAGAGGGATTAAAGGTGTGTTCTAAGTCAGAGCCAGAGTTTAATTAAAAACAGCTTTTTTTTTTCTTTAAAGCAAATAACATAAATCTCAGGGTTCACTGTGGGCCTGTGTACCATTTCACAGTGTCATCAAATATCCTGCAAGAGTAATGTGAATACCTGTGTTTTCCGATGGTCTTAAGCAACCCCTGTGAAAGGGTCAGTTGACAACCCCCACAAAGGGGTCAAGACCCACAGGTTGAGAACCATGGCTATAAGGAGACCAGTTGTTAGCTTAGAACTCACCCTGAGCCACTGTGAAGTCATTCTCACAGACAAGTAAGATCTTGTTAATGGGCATTAGACTTAGACTTCCTTTTGGAGGAAACAGCTTTTTCTCTTTCTGACCCAGTGGCCTTTATCTTCCCTTACCAAAAGGTGCTTACCTGCTCTGTTCACATTGGCATCCATCTTTGTCCCAAGTTCTAAGAGGTACAATGGATGGAAAAATGTGACAGCAGGATCCATAAGGTCACAGCCTGGTGTCTGAATCTTGTGATTTACATCATTCTATTAAAAACTTAGCAACTTGAATTCTGGCCCTGGGTTTTGACGATGGGAGATGGTGATTTGTGCGGGTGGTTCCACTTGTTACATGGCATCGCTTACGGTAACTCATTGGCTTGGTTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGATGGTGATTTGTGCGGGT
59.96
CCTGAATGGGAGGCACTGTT
59.96
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGATTTGTGCGGGTGGTTC
60.04
CCTGAATGGGAGGCACTGTT
59.96
157
PCR template sequence:
GAATTCTGGCCCTGGGTTTTGACGATGGGAGATGGTGATTTGTGCGGGTGGTTCCACTTGTTACATGGCATCGCTTACGGTAACTCATTGGCTTGGTTTGGTAAGTTAATGGTAAAAATGTTTTTATAGTAGTCCAGGTTTGCCTAGTCACTGTCGTGCTTGTCAGCTTCAAACAGTGCCTCCCATTCAGGTAACTACTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
29
Interacting miRNAs (#binding sites)
mmu-miR-709(2), mmu-miR-16-5p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-let-7e-5p(1), mmu-miR-203-3p(1), mmu-miR-125b-5p(1), mmu-miR-23a-3p(1), mmu-miR-128-3p(1), mmu-miR-503-5p(1), mmu-miR-125a-5p(1), mmu-miR-127-5p(1), mmu-miR-411-3p(1), mmu-miR-483-5p(1), mmu-miR-511-3p(1), mmu-miR-34b-5p(1), mmu-miR-297a-5p(1), mmu-miR-383-5p(1), mmu-miR-224-5p(1), mmu-miR-705(1), mmu-miR-29b-2-5p(1), mmu-miR-377-3p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-1198-5p(1), mmu-miR-466p-5p(1), mmu-miR-3065-5p(1), mmu-miR-490-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTGGCTTGGTTTGGTAAGTTAATGGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIATP8B1_2683_ORF_4
0.6292
-0.317079828
0.008687826
105
ORF sequence:
ATGGGAGATGGTGATTTGTGCGGGTGGTTCCACTTGTTACATGGCATCGCTTACGGTAACTCATTGGCTTGGTTTGGTAAGTTAATGGTAAAAATGTTTTTATAG
Peptide sequence:
MGDGDLCGWFHLLHGIAYGNSLAWFGKLMVKMFL
IRESfinder result
IRESfinder score
0.86894597
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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