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mmu_ciAsxl1_2322
Basic information of circular RNA
PanCircBase ID:
mmu_ciAsxl1_2322
mm39_Chr. Position ID:
chr2|153194191|153196513|+
Gene name:
Asxl1
Transcript:
ENSMUST00000227428.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
2322
Exon/intron sizes in nt:
2322
Flanking exon/introns:
chr2:153194191-153198989
Splice length:
2322
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGTATTGCTCTTACTGTTTAAATGACATATGTGCCTGTGAGAGTCATCTGTTGGTCTATCTGTGGTTGTGTATGTAATGGTAGTGTATCTGGTGTGTATTTTGAGGGTGGGAGTGGAGTGGGTAAACATCTGTTTGTTTACATCAGAAATACAGTCTTTCACAAGACCTTACCTAGGTCTTGACTTACAGCTAGAACTGGGTTCTTCTTTTCTTTTCTTTTCTTTCCTTTTTTTTATTTACATGAGTACGCTGTAGCTGTCTTCAGACACACCAGAAGAGGGCATCAGATCCCATTGCAGATGGTTGTGAGCCACTATGTGGTTGCTGGGAATTGAACTCAGGACCTCTATCAGTGCTCTTAACCGCTGAGCCATCTCTCCAGTCCGGAGTTCTAACTTTCTAATACAGTTTTGTTTTTTTGTTTTTGTCTTTTTTTATTTTTATTTTTATTTTTTTATTTTATTTTATTTTTGGTTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGCACTCACTCTGTAGACCAGGCTGGCCTCAAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGACTAAAGGCGTGCGCCACCACGCCCGGCTCGTTATTTTGTTTTTTAAGGTAAGGTCTCATTGCAGCTTTACCTTCTCGTAGACTCTTGTTTTCAAGTTTTGGTAATAGAGAAGGAACAAATATATATATTCTTTTCTCTATACTCTTTATACACAGGCCTGAAGTCTTTTCCTTTCTGATCCAAGAAAAATGGTCTCGGTGCCATTACACCCTCTATCTATCTACTCACTAATTTAGTCATTCACTCCTGTTTCTAAGTCTCTTGCAGCCCAGGTGGCTTCCAAATTGCTGCTCAGCTGAGGGCTTGCCTTGAATTCTGATCCTCATGTTTCTACCTTGCCATCCACAAGTAGCTAATTTAGCCAGTTGTGATGCAAATATACTAAGGAGTAATTTGTGGAATTTGATACAATGTAGTTTTCCTGGAGAACAAAAAAATACAGAAGTAATTGGAGGCATAAAAACCAGGGGTGCCTTTAATTCGGTTCTTTTCTCATCATTATACCAATTTCTCCTTTTTTACTTTTTTTTATTCATCTATATCTGTCTATCCATTTATTTTTTTTGAGACAGGATTTCCCTGTGTAGCACTGACTATCTTGGAATTTATTCTGTAGACCAAGTTGACCTCAACTCAGATCTACTTGCCTCTGCCTCCCAAATGCTTGAGTTAAAGTCATGTGCCACCTCTACCCAGTGAAAAAAACCTTTTTATTGTTATTGTGTGTGTTTTACACTAGTGTATGTCTAAATGGTGTGCATTTACTGCCTATGGAGGCCAGAAGAGGGCATTGGGACAACTGGAACTGAAATTACATACAGTTGTGAACTGCTAATGGGTACTGGAAATTTGAACCCAGGTCCTCTGGAAGAGCAGTTAATATTGTTCTTTTTTTTTTTTTTTTTTGGTTTTTCGAGACAGGGTTTCTCTGTGTAGCCCAGGCTGCCCTGGAATTCACTGTAGACCAGGCTGGTCTCGAACTCAGAAATCCACCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCGTGTACCACCACGCCTGGCCAGTTAATGCTTTTAACACCACTGAGCCACTACCTCAGCTATTTCCCCACCCACCTTTCTTTTTAATGTGAGCGAGGAAGGGGGAGTCCATAGAGTAGCAGTCCTTTTTTGGGGCTTGGAACAAACAGCATTTTGTCTGTGGTGGATATTGCATACATGCTTATGTATGTGCAGGGGCGTGGCGTGTGTTAGATCAGAGGACAACTTTGGATGTCATTCTTCCTTGCTTTTTGAGAAAAAGGGTCTCTTATGGGACCTACTGCTTGCAAATTGGGCTGTGCTGGTTTGCTACCAAGCCTGTCTCTGTCTCCCCAGCACCAGGACTGCAGGCGCCTGGCTACCATGCCTGCTCTAGGTTTTTTTTTTTGTTTGTTTGTTTGTTTGTTTTGTTTTGTTTTTTTAACATGTGGTTTTTGGGGTGTGAAGTCTGGTCTAAGTGTTTGCTTGAGAAGCACTTTATTGAAGGAGCCCTAAGCCCTAATTTCTCCTCAGCCCTAAGCAGTTAGTGTATTATATTGCTAATCACATTGTAGGCATTTAAAGTGAGAAGTTATTTCTCAGGTTGCATATTCTTTAGCTAATGTATTGTGGGATGGGATGCTTGGGGTTGCCTCCAGCCTATCTATTTGACTGTAGCTATGCACTTTACAGTGTGATAATTATGCTGTGTTTTTCCTCCCATGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTGTGGGATGGGATGCTTGG
60.32
CAGATGACTCTCACAGGCACA
59.73
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGTGGGATGGGATGCTTGG
60.32
ACAGATGACTCTCACAGGCAC
59.73
154
PCR template sequence:
TTGTGGGATGGGATGCTTGGGGTTGCCTCCAGCCTATCTATTTGACTGTAGCTATGCACTTTACAGTGTGATAATTATGCTGTGTTTTTCCTCCCATGTGGTTTGTATTGCTCTTACTGTTTAAATGACATATGTGCCTGTGAGAGTCATCTGTTGGTCTATCTGTGGTTGTGTATGTAATGGTAGTGTATCTGGTGTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
35
Interacting miRNAs (#binding sites)
mmu-miR-709(4), mmu-miR-298-5p(3), mmu-miR-20b-5p(2), mmu-miR-106a-5p(2), mmu-miR-93-5p(2), mmu-miR-20a-5p(2), mmu-miR-17-5p(2), mmu-miR-339-5p(2), mmu-miR-10b-5p(2), mmu-miR-539-5p(2), mmu-miR-23b-3p(1), mmu-let-7a-5p(1), mmu-miR-7b-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-322-5p(1), mmu-miR-106b-5p(1), mmu-miR-18a-5p(1), mmu-miR-7a-5p(1), mmu-miR-196a-5p(1), mmu-miR-320-3p(1), mmu-miR-23a-3p(1), mmu-miR-495-3p(1), mmu-let-7d-5p(1), mmu-miR-153-3p(1), mmu-miR-96-5p(1), mmu-miR-382-5p(1), mmu-miR-467b-5p(1), mmu-miR-183-5p(1), mmu-miR-383-5p(1), mmu-miR-466d-3p(1), mmu-miR-98-5p(1), mmu-miR-27a-5p(1), mmu-miR-369-3p(1), mmu-miR-466b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCCTCCCATGTGGTTTGTATTGCTC
siRNA sets
AATACAAACCACATGGGAG, ATACAAACCACATGGGAGG, CAATACAAACCACATGGGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.935224293
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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