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mmu_ciAscc3_1054
Basic information of circular RNA
PanCircBase ID:
mmu_ciAscc3_1054
mm39_Chr. Position ID:
chr10|50596806|50597860|+
Gene name:
Ascc3
Transcript:
ENSMUST00000035606.10
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
23
Exon/intron offsets:
0
Genomic length:
1054
Exon/intron sizes in nt:
1054
Flanking exon/introns:
chr10:50596806-50597868
Splice length:
1054
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGTACATATTTATGAGGCTATTCCGTTATTTTGCTACCATGAAGATAATCTGCCACTCATTTTATCTCTGTGATACCATTCCATTAAAGTTTTATTTACTATTATTTCTGCCTCTGTAGTGTTAAAGGAAGACAGTAATTAAATGTATGGTGTTGGTAAATCAATGTAATCATTGCTTGCAAACTTTAATTATCATTTGGTTATGTCTTCCAGGATTGTAGAATATGAAACTAGGTGAAATAAGTAATGAATAGGTGATATATACATAGAGTAGTAAGAACATTGAATCACATTGTGAAAATTCTTTACAAATCATTCATCAGCCCTGAAAAATACCTGCAAGTAGTAGTATATGCACTAAACAGGTTACATTTATATATTTTGGAATGTTTGCAGACATACATGCACGCACGCATGCACACATATGCATGTAAGAACAACTGATGAGAAAAGAGGCCATGGATTTTAAAGAGCACAAGGAGTATATGGGAAGGTGTTGAGAGAGTTAAAGGGGGAGAGGTAAATGAAGTTAACTATGTTAGAATCTCAAAAATAATTGGAATAATTTTTTATTAGATATTAAAGAGAAAAAGGAGAGCTTTATGCTGAACAACCTTATGTGAGCACCAGATATGTTTTTCTTGCATTTATTTTTAATTTAAACATATTTGTTACAAGCAATTGTGGAATTTCTCTTAAAGTACTGGTAGACTTAAACAGAAACATTGGGGAAATATTAGTATAATAATTAAATTTTTTAAAATGAAGTTAAGTAAATTGAATAGTGTTTGGGTAATTTTGATACATGAATCAAAGTGATAACCTGTAAATACATGGTAAATAACACAAAATGAATATAAACTTTAATCCACGGCCTATTAGGTTCTTAAAGGGAGTTGCACACAAGATGCTGAAATACCTAAGCATCTCAGCTCTTTAAAGTCCAGTGTACTTCTTTGTCACTACATCTTGTCTAGGTATCGCTTTCTTAACAGTAGACTACTGTGAACCTCTACAGTCGTAAAAGACATACTGACTTGAGACTTTATTT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACATCTTGTCTAGGTATCGCT
55.98
TGGCAGATTATCTTCATGGTAGCA
59.9
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACATCTTGTCTAGGTATCGCT
55.98
TGAGTGGCAGATTATCTTCATGGT
59.84
149
PCR template sequence:
CTTCTTTGTCACTACATCTTGTCTAGGTATCGCTTTCTTAACAGTAGACTACTGTGAACCTCTACAGTCGTAAAAGACATACTGACTTGAGACTTTATTTGTAAGTACATATTTATGAGGCTATTCCGTTATTTTGCTACCATGAAGATAATCTGCCACTCATTTTATCTCTGTGATACCATTCCATTAAAGTTTTATTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-709(1), mmu-miR-191-5p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-297a-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-300-3p(1), mmu-miR-539-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGAGACTTTATTTGTAAGTACATATT
siRNA sets
TATGTACTTACAAATAAAG, ATGTACTTACAAATAAAGT, AATATGTACTTACAAATAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.879374243
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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