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mmu_ciArl1_100

Basic information of circular RNA
PanCircBase ID:

mmu_ciArl1_100

mm39_Chr. Position ID:

chr10|88569542|88569642|+

Gene name:

Arl1

Transcript:

ENSMUST00000170137.8

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

3

Exon/intron offsets:

0

Genomic length:

100

Exon/intron sizes in nt:

100

Flanking exon/introns:

chr10:88569542-88571759

Splice length:

100

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTAAGTATTGGTTTAATAACACTTTTCATGTTTCTATCTAATACAGCTTTCTGAATAAACGAGTCATATGATGGGGGTTTGTAAACTTCTACTATGGAAA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

ACGAGTCATATGATGGGGGT

57.9

AGAAACATGAAAAGTGTTATTAAACCA

56.68

77

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

ACGAGTCATATGATGGGGGT

57.9

GAAACATGAAAAGTGTTATTAAACCA

55.46

76

PCR template sequence:

CTGAATAAACGAGTCATATGATGGGGGTTTGTAAACTTCTACTATGGAAAGTAAGTATTGGTTTAATAACACTTTTCATGTTTCTATCTAATACAGCTTT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

1

Interacting miRNAs (#binding sites)

mmu-miR-320-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TCTACTATGGAAAGTAAGTATTGGTT

siRNA sets

AACCAATACTTACTTTCCA,AATACTTACTTTCCATAGT,CAATACTTACTTTCCATAG

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIARL1_100_ORF_1

0.9233

-0.590994069

0.004872556

87

ORF sequence:

ATGATGGGGGTTTGTAAACTTCTACTATGGAAAGTAAGTATTGGTTTAATAACACTTTTCATGTTTCTATCTAATACAGCTTTCTGA

Peptide sequence:

MMGVCKLLLWKVSIGLITLFMFLSNTAF

IRESfinder result

IRESfinder score

0.878186699

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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