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mmu_ciArfgef2_125
Basic information of circular RNA
PanCircBase ID:
mmu_ciArfgef2_125
mm39_Chr. Position ID:
chr2|166702318|166702443|+
Gene name:
Arfgef2
Transcript:
ENSMUST00000099078.10
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
17
Exon/intron offsets:
0
Genomic length:
125
Exon/intron sizes in nt:
125
Flanking exon/introns:
chr2:166702318-166702444
Splice length:
125
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAAATGCCCCATGGTTTGTATAGCGTGGCTGCAGACATGGCTGAATTTGTTAGGGCATTTCAGTGTTTTAAAAATGTTTTTCTTTTTTTCCTTTTTCTTTTTTTCTTTCTTTTTTCAATTTCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
NA
NA
NA
NA
NA
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
NA
NA
NA
NA
NA
PCR template sequence:
AGTGTTTTAAAAATGTTTTTCTTTTTTTCCTTTTTCTTTTTTTCTTTCTTTTTTCAATTTCAGTAAAATGCCCCATGGTTTGTATAGCGTGGCTGCAGACATGGCTGAATTTGTTAGGGCATTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-495-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTTTCAATTTCAGTAAAATGCCCCA
siRNA sets
TTTTACTGAAATTGAAAAA, ATTTTACTGAAATTGAAAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIARFGEF2_125_ORF_1
0.4841
-0.597400572
0.006447076
243
ORF sequence:
ATGCCCCATGGTTTGTATAGCGTGGCTGCAGACATGGCTGAATTTGTTAGGGCATTTCAGTGTTTTAAAAATGTTTTTCTTTTTTTCCTTTTTCTTTTTTTCTTTCTTTTTTCAATTTCAGTAAAATGCCCCATGGTTTGTATAGCGTGGCTGCAGACATGGCTGAATTTGTTAGGGCATTTCAGTGTTTTAAAAATGTTTTTCTTTTTTTCCTTTTTCTTTTTTTCTTTCTTTTTTCAATTT
Peptide sequence:
MPHGLYSVAADMAEFVRAFQCFKNVFLFFLFLFFFLFSISVKCPMVCIAWLQTWLNLLGHFSVLKMFFFFSFFFFSFFFQF
IRESfinder result
IRESfinder score
0.990869942
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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