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mmu_ciArfgap2_295

Basic information of circular RNA
PanCircBase ID:

mmu_ciArfgap2_295

mm39_Chr. Position ID:

chr2|91100634|91100929|+

Gene name:

Arfgap2

Transcript:

ENSMUST00000080008.13

CircRNA type:

Circular intronic (ci)RNA

Exon/Intron count:

1

Exon/intron indexes:

10

Exon/intron offsets:

0

Genomic length:

295

Exon/intron sizes in nt:

295

Flanking exon/introns:

chr2:91100634-91103885

Splice length:

295

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GTGAGGCTCGGGCCCTGGTGTGTTAGAAAAATGCCATTGTTTTTCTGGGAGCCAGAACAGGCTCTACCCAGTCAGTCAGGGTTCCTTGCTTAGTCTTCCTCTGAGGCTAAAGACAGAAAATGTTGTTGTTTTAAAGTACCTTGGACAGTGGTTGGCCACACGTGTTAGTTGCCTAAGTTTTAAGAGAGCAGGATCAGGTGCTGTTTTTTTACTCCAGGTTAGGACGGCACTTATTAATATGGCTGCTTTGTGACTGAGCAGTTTTTTTCCCCAAGACCTGAGAACCTTAGGGCTT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TACTCCAGGTTAGGACGGCA

59.96

ACTGACTGGGTAGAGCCTGT

59.88

162

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TACTCCAGGTTAGGACGGCA

59.96

GCAAGGAACCCTGACTGACT

59.6

175

PCR template sequence:

AGGTGCTGTTTTTTTACTCCAGGTTAGGACGGCACTTATTAATATGGCTGCTTTGTGACTGAGCAGTTTTTTTCCCCAAGACCTGAGAACCTTAGGGCTTGTGAGGCTCGGGCCCTGGTGTGTTAGAAAAATGCCATTGTTTTTCTGGGAGCCAGAACAGGCTCTACCCAGTCAGTCAGGGTTCCTTGCTTAGTCTTCCT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

3

Interacting miRNAs (#binding sites)

mmu-miR-7a-5p(1), mmu-miR-350-3p(1), mmu-miR-490-3p(1)

siRNAs for circular RNA silencing
Junction sequence

AACCTTAGGGCTTGTGAGGCTCGGGC

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.731460627

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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