top of page
mmu_ciAls2_412
Basic information of circular RNA
PanCircBase ID:
mmu_ciAls2_412
mm39_Chr. Position ID:
chr1|59224758|59225170|-
Gene name:
Als2
Transcript:
ENSMUST00000027178.13
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
17
Exon/intron offsets:
0
Genomic length:
412
Exon/intron sizes in nt:
412
Flanking exon/introns:
chr1:59224758-59225170
Splice length:
412
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTAGTGATTGCTTCTCTCTTGGCCTTTTCTGTCTTTTATGTTAGTAAAAAAAATAAATACTGTTGCTTCATTAATAACAATTAAATCAAAACTAGACTTGCCTTTTTTGTTTTCCCGAAACATAGTCTTGTCGTGTAGCCCTAGCTAGCCTTGAATTCTTTCTGCCTTAACCTCAAGTGTCAGGAATACTCCAGGATGCCCGATTGTCCTTACTGAATTGTTTGTTTGTTTATTCACTCACTCATTCATTTATTGGTAGATCTTCCTCTGTGGTCCAGGCGAGCTGGTTCACATCTCTCAATAAGCACAGTGATAGGCAATAGAAAAAGTACTGCAGAAGAGCCAGCAATACTAGTATAGGCTTGATGACTTATTGCTTTAAACTTGCTGACATGGGCTTTTTGCTTCTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGCAATAGAAAAAGTACTGCAGA
58.44
AACATAAAAGACAGAAAAGGCCA
56.87
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTACTGCAGAAGAGCCAGCA
60.04
TTTAATTGTTATTAATGAAGCAACAGT
55.18
169
PCR template sequence:
GATAGGCAATAGAAAAAGTACTGCAGAAGAGCCAGCAATACTAGTATAGGCTTGATGACTTATTGCTTTAAACTTGCTGACATGGGCTTTTTGCTTCTAGGTTAGTGATTGCTTCTCTCTTGGCCTTTTCTGTCTTTTATGTTAGTAAAAAAAATAAATACTGTTGCTTCATTAATAACAATTAAATCAAAACTAGACTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-322-5p(1), mmu-miR-690(1)
siRNAs for circular RNA silencing
Junction sequence
TTTTTGCTTCTAGGTTAGTGATTGCT
siRNA sets
AATCACTAACCTAGAAGCA, ATCACTAACCTAGAAGCAA, CAATCACTAACCTAGAAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.946194501
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page