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mmu_ciAlox12_61
Basic information of circular RNA
PanCircBase ID:
mmu_ciAlox12_61
mm39_Chr. Position ID:
chr11|70143775|70143836|-
Gene name:
Alox12
Transcript:
ENSMUST00000000329.9
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
4
Exon/intron offsets:
0
Genomic length:
61
Exon/intron sizes in nt:
61
Flanking exon/introns:
chr11:70143724-70143836
Splice length:
61
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGGAGAGGGTGGACGCTCATGGGATGGGGTCTGGGAGATCCACGAATGGAGGTGGACA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTGGGAGATCCACGAATG
57.77
CCATCCCATGAGCGTCCAC
60.52
60
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTGGGAGATCCACGAATG
57.77
CCATCCCATGAGCGTCCA
59.09
60
PCR template sequence:
GTCTGGGAGATCCACGAATGGAGGTGGACAGTAAGGAGAGGGTGGACGCTCATGGGATGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGGAGGTGGACAGTAAGGAGAGGGT
siRNA sets
CTCTCCTTACTGTCCACCT, CTCCTTACTGTCCACCTCC, CCTCTCCTTACTGTCCACC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIALOX12_61_ORF_1
0.7136
0.240765239
0.097954335
99
ORF sequence:
ATGGGATGGGGTCTGGGAGATCCACGAATGGAGGTGGACAGTAAGGAGAGGGTGGACGCTCATGGGATGGGGTCTGGGAGATCCACGAATGGAGGTGGA
Peptide sequence:
MGWGLGDPRMEVDSKERVDAHGMGSGRSTNGGG
IRESfinder result
IRESfinder score
0.14659649
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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