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mmu_ciActr1a_667
Basic information of circular RNA
PanCircBase ID:
mmu_ciActr1a_667
mm39_Chr. Position ID:
chr19|46372784|46373451|-
Gene name:
Actr1a
Transcript:
ENSMUST00000040270.6
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
667
Exon/intron sizes in nt:
667
Flanking exon/introns:
chr19:46372784-46373451
Splice length:
667
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAATTCCCCGTCCAACAGGAGAAGTCTCGGGCTTGCAGAGAGACCACCACAGAGAACATAGCAATGAGGAAAGGGGTTGTTTTTCTCCCAAGAGCAGACTCTGACCTTAAAGAAGACATATCTGGAACCCCACATGGTGGGGCAGAGACCAGTCTAGAAAAGGACACTGTTATTCTGTGTCCTGTCGCTGTCTTGCAGTCATTAGATTGATTGGCTTGGGTATGTTTTTGGCAAGAGGAATTCAGAGGGGAAATCCAATCTGGCCTATCCAAGGTATAAGTTCATTACCCTAGCTTTGGCAAAGCCCTCAGACAGGCTCTAGGAAACATAACTACAGAGCCATTGCTGCCCATTTTGGTTCCAAGAACTTAGGTAAGAGCCAGGACACTGCGCCCCAGAACCAGGCAACAAGGCGGGCACACAGGGCCTGGGGGTCATGGGAATCGGGAAGCCTCTGCTGCTTGGCTGCTCACTCGGAGAGTCCAGCAGCATCCTGTAGCATCCAGCCTCCAACTTAGAGAGAGAATGCCTACACTCAGTGAGGGCTGGGACCCGGGAGGCTGAGGTTCCTTGACCCCCGGGAGATTCTGCCTGTACCCTCGGGCTGGGAGGGCAGGATGCCAGGCAGAGCCTGACTCTGCTACAGACTATGCCCTCTACTTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGGGAGATTCTGCCTGTACC
59.89
CTCTGTGGTGGTCTCTCTGC
59.75
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCGGGAGATTCTGCCTGTAC
59.89
CTCTGTGGTGGTCTCTCTGC
59.75
144
PCR template sequence:
TTCCTTGACCCCCGGGAGATTCTGCCTGTACCCTCGGGCTGGGAGGGCAGGATGCCAGGCAGAGCCTGACTCTGCTACAGACTATGCCCTCTACTTGCAGGTAATTCCCCGTCCAACAGGAGAAGTCTCGGGCTTGCAGAGAGACCACCACAGAGAACATAGCAATGAGGAAAGGGGTTGTTTTTCTCCCAAGAGCAGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-384-3p(1), mmu-miR-190b-5p(1), mmu-miR-207(1), mmu-miR-484(1), mmu-miR-540-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTCTACTTGCAGGTAATTCCCCGTC
siRNA sets
GAATTACCTGCAAGTAGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.625147245
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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