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mmu_ciAcap2_322
Basic information of circular RNA
PanCircBase ID:
mmu_ciAcap2_322
mm39_Chr. Position ID:
chr16|30929388|30929710|-
Gene name:
Acap2
Transcript:
ENSMUST00000230698.2
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
14
Exon/intron offsets:
0
Genomic length:
322
Exon/intron sizes in nt:
322
Flanking exon/introns:
chr16:30929388-30929710
Splice length:
322
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGTGTATGCTCAGCGGGAGTGGAGCGCTCCTTTGGGTTAAATACAGGTTGCAAATTATTGTACGGGTTTTTGTTGTTGCTGTTTTTGTTAGTGAGTCTTTTTTCCCAGTTTTCTAATTTAATTACTAGTTTATAGTTGCACATTGTAACCAATGGATAATTTTAGTTTGTAATGTGTTTTTGATGACTTTGGTTCATCATTCATTAATGACCACAGAGGATCCATCCATATTTATGTAGGTGCCATCTGTGTGTGAGTAAATGCACAAATAGTTGCTTGATATTTTTATATAAGAATAATGCTATCTTTTTCCCTATGTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGTGCCATCTGTGTGTGAG
59.96
ACAGCAACAACAAAAACCCGT
59.73
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGCCATCTGTGTGTGAGT
60.25
ACAGCAACAACAAAAACCCGT
59.73
166
PCR template sequence:
CCATCCATATTTATGTAGGTGCCATCTGTGTGTGAGTAAATGCACAAATAGTTGCTTGATATTTTTATATAAGAATAATGCTATCTTTTTCCCTATGTAGGTGTGTATGCTCAGCGGGAGTGGAGCGCTCCTTTGGGTTAAATACAGGTTGCAAATTATTGTACGGGTTTTTGTTGTTGCTGTTTTTGTTAGTGAGTCTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-183-5p(1), mmu-miR-342-3p(1), mmu-miR-377-3p(1), mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTCCCTATGTAGGTGTGTATGCTCA
siRNA sets
TACACACCTACATAGGGAA, CATACACACCTACATAGGG, ATACACACCTACATAGGGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIACAP2_322_ORF_1
0.8208
-0.580768046
0.006971952
159
ORF sequence:
ATGCTATCTTTTTCCCTATGTAGGTGTGTATGCTCAGCGGGAGTGGAGCGCTCCTTTGGGTTAAATACAGGTTGCAAATTATTGTACGGGTTTTTGTTGTTGCTGTTTTTGTTAGTGAGTCTTTTTTCCCAGTTTTCTAATTTAATTACTAGTTTATAG
Peptide sequence:
MLSFSLCRCVCSAGVERSFGLNTGCKLLYGFLLLLFLLVSLFSQFSNLITSL
IRESfinder result
IRESfinder score
0.909525592
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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