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mmu_ciUbn2_3235
Basic information of circular RNA
PanCircBase ID:
mmu_ciUbn2_3235
mm39_Chr. Position ID:
chr6|38464085|38467320|+
Gene name:
Ubn2
Transcript:
ENSMUST00000160583.8
CircRNA type:
Circular intronic (ci)RNA
Exon/Intron count:
1
Exon/intron indexes:
13
Exon/intron offsets:
0
Genomic length:
3235
Exon/intron sizes in nt:
3235
Flanking exon/introns:
chr6:38464085-38467318
Splice length:
3235
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGAGTCCAGCAAATCTATAGTTGGTCCTAATTGGTGAGTGTTGGCATGGATTTTTAAGATGAAAAGAACATATGCTGTTTAGCAAAGATTAATAGATGCTAAATTCCTTGATTTCCATGTCATAAAAGTCCATTTGAAAAATAGATTCTTGTGGAGCAGTGGGAATTTGCATACATTGACTCTATATAGAAATATTGGATTAAATAAAACAAATGCTCTTTAAAAACATAGTTGTCTGGACATAGTTGTATGTGCCTTTAATCCCAGCCCTCAGGAGGCAGAGGCAGGCATATCTCTGTGAGTTCAAGGCCAGCCTGGTCTACAGAGATAGTTCTAGCATAGCTAGGGCTACATAGAGAAACTCTGTTCCAAAAAACATAGCTAAGATTGTAAATAGAAAGGAGAAAATCCAGAGTCAAAGAGAAGCTACCCAGTTGATCTGTTGCTGAGGCCTCATTGCCCTTGGAAAGACTACCAATCACTTTATGGAGAGACTACATATAGAGTGAGACATGGTTTCTTTTCACTTTATTTTCAGAGTATAGCAGGTAGGATTTTCTGGGGATAGTATAAGTCAGAAATAGAAAAGAAGCCAGTGTAAAACAGGGCCCTGAGGGGGATTGAGAGATGGCTCAGTGGTTCTTCTAAAGGTCCTGAGTTCAATTCCCAGCAACTACGTGGTAGCTCACAACCATCTGTAATAGGATCTGATGCCCTCTTCTGGTGTGTCTGAAACAACAGTCTACTCACATACATAAAATAAATAAATCTTTAAAAAACAAAAAGCAAAAAAACAGTGCTTTGAAAGAATTCCATCTTTAGTTACAGATGAGGAGAAGAGGGAGCAGTAGAGAAATGAAGAGTTATGTCTTGGCCAAGGGTCTAGATTAGAGAAGAACAGTCCTGCCTGAAAATGTCCCATGTGTTAGGACTTTAATTTGCATTACTTGGCAAAGCCTTACAGGTGGCCTAGGACAGCAGATAAAAACAGGTTTTGTGCCATGTAATGTTTCACATCCTAGGCTTGCTTACTTGTCAGTCAAACATAGGCTATAGACAGAGCTCAGACAGAGTGTAGACTTTGCATGGCAGAGGTCTGTGTTTAATCTTACCACCACAGAAAAGACCCAAGATAATAATAAAAATAAATAAATAAAACATAACAAAAAAAACAAACAACAAACAAGAAAAGCTATGAGCTTACTATCTACTGTTTGGAATATAAACTACATGGCAGGGGTACATTAGTATAGGCTAGAATCAGTGGACACCACAAACAATTAAATCCCCAAGAGGACAGGCCATAGGTTTATTAATTTAGAACATAAAATTGCCTGGACATAGTGGTACTAATCTGTAATCCCAGAACTCTGGAGGGAGAGGCAGAAGAATTGCAGCAAGTTTGGGACCAGTCTGTCTTCTACATAACTCCAGGTCACTCAGGGATACATAGACCTGCTCTTACCTCCAAGAAAAAAGAACATATTGACAGTGCTAAGGAGAATCTGTGAGCCCTATCACATTTCATCTGTTCTTTAGCCAGGAACAAGTCTTCCTTCACTACCAAAGTGACTATTTAAGGTACAAAGGCAGACATATCCATAACATAGGTAGGGCTCATTTGATAAGTGCTTGCTCTAGCATCCTTGAAGCCCTCACATCATATAAAGCAGTCATGGTAGTGCATGACTGTAATCTCAGCACTCAGTATGTGGAGACAGTAGCATCAGAAGTTCAAGCTGACTCTTGACTTGATGGAGTTGGAGGTTAACTTGGGATAAAAAAGACTCTGCCTCCCCCAAAAGAGATAATTAAAATAGAAACTTTGTGCTGGTGATACCTTTGATATTATTGCCCCTACCTCAGTCCTTTACTCTCTAAAGATGAAGTTTTAGTAATAGTGTGCTTGGACTGTCAACATGTGCAAGCATGTGTCCATGTCTACCCCTCATCTGAGCAAGCAGTGGAGCCTAATGGGGTCCTACCCACACTACTATAATAGTGAGGCTTTTACATTGTTGGAATAAAAGTGTAGGACAAGTGCTATGGAAGTCAGAGTGTGACAGCATTCTTAACAGCTTACTTACTGTAACACCGTAGGCGGTGCTGGTAAAGACCCTTCCTGTTCGTTCTTTTCCCACTATGCTGAAGGTAAGTGATGTGGCTGTATACATCAGTATATAAGACTCTGGGTTTGGGACTTTTTCATCCTAAGGAGCTAGTAGCACCTCTGATGATCTGTGCAGTAGTTATGTCTTTATCTTAAACATGAAATCTAGCACTTTATATTACTATCAACAAATAAATGGTTGCTCTTTAACTTGCTAATATTTTTTCTCTGACTATTTTAAAATATAATTCTCTTAACTCTTCCTTGAATTTTAAGAAAATTAATTTAACAGACAGTATTCACTTAGATGTTTAGCATTTTAATTATTGATACCATCATATTCTTAGGACAGTAAAACTTAGACTTAAGATTTAGACGAGTGTCTTCTCACTTGTAGGAGACAGTAAGCACATACTTGATACGTTACAAATGATGTCAGTCAAAATTAGTAGAATGATTTAAATATGGTTGAGTAACTCACTGGTTTCCTAGTAGAGTGGTGGTTGGGAGCTGTAGAAATACAAGGGAGCAGAGAGCAAGAGTTAGCACCCCAGCAAACCTTATTAGACAAGAGTCATAGTCTTGATGTAGAAATGAAGTCCCTTCCCAGCACCAGGACAGTGAAAGTGTCTGATAAACCGGGATGGACATAGGTTCTGAACTTCTAATTTAAGTGGAGTTAACGAGTGATGTCAGTTAGTATCCTTTAGCCCCATTTTCAGGTCCTGGTAAGATGACTTGCAAGGACATGGTGGTGTAGTGTGTCACACACTGTTACTTGGAGAGCGGGTAGAGTACTGTCTTAATGTTATTTTGGTTTTGGCAGTCTTTTCAAGATTTCTTGCTTTAAAATAAAGGGCCCAGTACTGTGGCATATGATTTTTATATCAGTACTTGGGAGGCAGAGGCACATATATCTTTGTGAGTTCAAGGTCAGCCTAGTCTACAGAAGGAGTACCAAAACAGGCAAAGCTATAAACAGACCTATATCAGAACAAACAAAATAACCAAAAATTAATAATTTTAGTCATCATAAGAATTTCTTAGCCTTTTAAAAACTAAATTTAAAATTTGTAAATTAACTGTATTTTTTTTTCTAATATAATGCTACTTATGCTAGGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGTCATCATAAGAATTTCTTAGCCT
56.09
TCCATGCCAACACTCACCAA
59.82
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTCATCATAAGAATTTCTTAGCCT
56.09
ATCCATGCCAACACTCACCA
59.59
151
PCR template sequence:
TTTAGTCATCATAAGAATTTCTTAGCCTTTTAAAAACTAAATTTAAAATTTGTAAATTAACTGTATTTTTTTTTCTAATATAATGCTACTTATGCTAGGAGTAAGAGTCCAGCAAATCTATAGTTGGTCCTAATTGGTGAGTGTTGGCATGGATTTTTAAGATGAAAAGAACATATGCTGTTTAGCAAAGATTAATAGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
30
Interacting miRNAs (#binding sites)
mmu-miR-5101(3), mmu-miR-101a-3p(2), mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-7b-5p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-let-7e-5p(1), mmu-miR-150-5p(1), mmu-miR-200c-3p(1), mmu-miR-26a-5p(1), mmu-miR-106b-5p(1), mmu-miR-7a-5p(1), mmu-miR-709(1), mmu-miR-203-3p(1), mmu-miR-125b-5p(1), mmu-miR-26b-5p(1), mmu-miR-494-3p(1), mmu-miR-342-5p(1), mmu-miR-1187(1), mmu-miR-344d-3p(1), mmu-miR-190b-5p(1), mmu-miR-3087-3p(1), mmu-miR-3470b(1), mmu-miR-670-5p(1), mmu-miR-323-3p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTTATGCTAGGAGTAAGAGTCCAGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.82779699
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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