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mmu_circZfp638_668
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp638_668
mm39_Chr. Position ID:
chr6|83935165|83946221|+
Gene name:
Zfp638
Transcript:
ENSMUST00000204751.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
14,15,16,17,18,19,20
Exon/intron offsets:
0,1747,3793,7345,8263,9109,10933
Genomic length:
11056
Exon/intron sizes in nt:
6,93,81,32,12,94,21,35,000
Flanking exon/introns:
chr6:83934574-83935165|chr6:83946221-83949227
Splice length:
668
Other database IDs:
circAtlas: mmu-Zfml_0072
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
CAAACTCTGAAATCAAGGCTAGTTCAGAAGACAAAGCCACTGGAAAGAGTGCTGAAGAAAGTCCTTCAGGTACTTTGGAGGCCACAGAAAAGGAACCAGTGAACAAGGAATCGGAGGAAATGAGTGTAGTATTTATTTCTAATTTGCCTAACAAAGGATATTCTACAGAAGAAATTTACAACTTAGCAAAACCATTTGGTGCTTTGAAGGACATTTTGGTTTTATCATCTCATAAAAAGGCATATATAGAAATAAACAAAAAATCTGCAGATTCTATGGTAAAATTTTACACCTGCTTCCCAATATCCATGGATGGAAATCAGCTCTCCATAAGTATGGCTCCTGAGCATGTGGACTTAAAAGATGAGGAAGCTCTCTTTACAACTTTGATTCAAGAAAATGACCCAGAGGCAAATATTGATAAAATTTACAACCGATTTGTACATCTTGACAATTTACCTGAAGATGGACTTCAGTGTGTGCTTTGTGTTGGGCATCAGTTTGGAAAAGTGGATCGCTATATGTTCATGAGTAACAAAAACAAGGTGATCCTTCAGTTAGAAAGTCCTGAATCTGCTCTGTCAATGTACAATTTTTTGAAACAAAATCCACAAAACATAGGTGAGCACGTGTTGACCTGCACTTTATCTCCAAAGACTGACTCAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TAGGTGAGCACGTGTTGACC
59.97
TGGTTCCTTTTCTGTGGCCT
59.44
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TAGGTGAGCACGTGTTGACC
59.97
TCCTTTTCTGTGGCCTCCAA
59.15
144
PCR template sequence:
TGAATCTGCTCTGTCAATGTACAATTTTTTGAAACAAAATCCACAAAACATAGGTGAGCACGTGTTGACCTGCACTTTATCTCCAAAGACTGACTCAGAGCAAACTCTGAAATCAAGGCTAGTTCAGAAGACAAAGCCACTGGAAAGAGTGCTGAAGAAAGTCCTTCAGGTACTTTGGAGGCCACAGAAAAGGAACCAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-181b-5p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
GACTGACTCAGAGCAAACTCTGAAAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP638_668_ORF_1
0.8055
-0.028590482
0.690943104
651
ORF sequence:
ATGAGTGTAGTATTTATTTCTAATTTGCCTAACAAAGGATATTCTACAGAAGAAATTTACAACTTAGCAAAACCATTTGGTGCTTTGAAGGACATTTTGGTTTTATCATCTCATAAAAAGGCATATATAGAAATAAACAAAAAATCTGCAGATTCTATGGTAAAATTTTACACCTGCTTCCCAATATCCATGGATGGAAATCAGCTCTCCATAAGTATGGCTCCTGAGCATGTGGACTTAAAAGATGAGGAAGCTCTCTTTACAACTTTGATTCAAGAAAATGACCCAGAGGCAAATATTGATAAAATTTACAACCGATTTGTACATCTTGACAATTTACCTGAAGATGGACTTCAGTGTGTGCTTTGTGTTGGGCATCAGTTTGGAAAAGTGGATCGCTATATGTTCATGAGTAACAAAAACAAGGTGATCCTTCAGTTAGAAAGTCCTGAATCTGCTCTGTCAATGTACAATTTTTTGAAACAAAATCCACAAAACATAGGTGAGCACGTGTTGACCTGCACTTTATCTCCAAAGACTGACTCAGAGCAAACTCTGAAATCAAGGCTAGTTCAGAAGACAAAGCCACTGGAAAGAGTGCTGAAGAAAGTCCTTCAGGTACTTTGGAGGCCACAGAAAAGGAACCAGTGA
Peptide sequence:
MSVVFISNLPNKGYSTEEIYNLAKPFGALKDILVLSSHKKAYIEINKKSADSMVKFYTCFPISMDGNQLSISMAPEHVDLKDEEALFTTLIQENDPEANIDKIYNRFVHLDNLPEDGLQCVLCVGHQFGKVDRYMFMSNKNKVILQLESPESALSMYNFLKQNPQNIGEHVLTCTLSPKTDSEQTLKSRLVQKTKPLERVLKKVLQVLWRPQKRNQ
IRESfinder result
IRESfinder score
0.769158465
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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