top of page
mmu_circZfp462_405
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp462_405
mm39_Chr. Position ID:
chr4|55050185|55051327|+
Gene name:
Zfp462
Transcript:
ENSMUST00000098070.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
8,9
Exon/intron offsets:
0,1005
Genomic length:
1142
Exon/intron sizes in nt:
2,68,137
Flanking exon/introns:
chr4:55023606-55050185|chr4:55051327-55060013
Splice length:
405
Other database IDs:
circBase: mmu_circ_0011687, CIRCpedia: MMU_CIRCpedia_23038, circAtlas: mmu-Zfp462_0001
circBase: hsa_circ_0137651, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_62126
Human Ortholog IDs:
Mature spliced Sequence:
ACTCCTCTTACTCGGAGCCCCCCGATGTTCAGCAGCAGCTGAATCACTACCAGTCAGCCGCCCTGGCAAGAAACAAGAGCCGCGTTAGCCCTGTGCCTCCTTCTGGGACTGCTGCTGGCACGGAACAGAAGGCAGAAGCCGTTCTTCACTGCGAATTCTGTGAATTCTCCTCTGGCTACATCCAGAGCATCAGGCGCCATTACCGGGACAAGCACGGTGGGAAGAAGCTTTTCAAATGCAAAGACTGCTCCTTTTACACCGGCTTTAAATCTGCTTTTACTATGCACGTGGAAGCTGGGCATTCGGCGGTCCCGGAGGAAGGCCCCAAAGATCTCCGCTGTCCTCTCTGCCTCTATCACACCAAATACAAACGCAACATGATCGACCACATAGTGCTGCACCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAAGGCCCCAAAGATCTCC
60.11
GCGGCTGACTGGTAGTGATT
60.11
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAGGAAGGCCCCAAAGATC
60.11
GCGGCTGACTGGTAGTGATT
60.11
153
PCR template sequence:
GCGGTCCCGGAGGAAGGCCCCAAAGATCTCCGCTGTCCTCTCTGCCTCTATCACACCAAATACAAACGCAACATGATCGACCACATAGTGCTGCACCGAGACTCCTCTTACTCGGAGCCCCCCGATGTTCAGCAGCAGCTGAATCACTACCAGTCAGCCGCCCTGGCAAGAAACAAGAGCCGCGTTAGCCCTGTGCCTCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-709(1), mmu-miR-320-3p(1), mmu-miR-326-3p(1), mmu-miR-153-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTGCTGCACCGAGACTCCTCTTACTC
siRNA sets
TAAGAGGAGTCTCGGTGCA, AGAGGAGTCTCGGTGCAGC, AGTAAGAGGAGTCTCGGTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP462_405_ORF_1
0.9012
0.34105561
0.834586965
528
ORF sequence:
ATGCACGTGGAAGCTGGGCATTCGGCGGTCCCGGAGGAAGGCCCCAAAGATCTCCGCTGTCCTCTCTGCCTCTATCACACCAAATACAAACGCAACATGATCGACCACATAGTGCTGCACCGAGACTCCTCTTACTCGGAGCCCCCCGATGTTCAGCAGCAGCTGAATCACTACCAGTCAGCCGCCCTGGCAAGAAACAAGAGCCGCGTTAGCCCTGTGCCTCCTTCTGGGACTGCTGCTGGCACGGAACAGAAGGCAGAAGCCGTTCTTCACTGCGAATTCTGTGAATTCTCCTCTGGCTACATCCAGAGCATCAGGCGCCATTACCGGGACAAGCACGGTGGGAAGAAGCTTTTCAAATGCAAAGACTGCTCCTTTTACACCGGCTTTAAATCTGCTTTTACTATGCACGTGGAAGCTGGGCATTCGGCGGTCCCGGAGGAAGGCCCCAAAGATCTCCGCTGTCCTCTCTGCCTCTATCACACCAAATACAAACGCAACATGATCGACCACATAGTGCTGCACCGA
Peptide sequence:
MHVEAGHSAVPEEGPKDLRCPLCLYHTKYKRNMIDHIVLHRDSSYSEPPDVQQQLNHYQSAALARNKSRVSPVPPSGTAAGTEQKAEAVLHCEFCEFSSGYIQSIRRHYRDKHGGKKLFKCKDCSFYTGFKSAFTMHVEAGHSAVPEEGPKDLRCPLCLYHTKYKRNMIDHIVLHR
IRESfinder result
IRESfinder score
0.480972585
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page