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mmu_circZfp160_358
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp160_358
mm39_Chr. Position ID:
chr17|21231937|21241096|+
Gene name:
Zfp160
Transcript:
ENSMUST00000232473.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,8396,9030
Genomic length:
9159
Exon/intron sizes in nt:
102,127,129
Flanking exon/introns:
chr17:21229248-21231937|chr17:21241096-21261684
Splice length:
358
Other database IDs:
CIRCpedia: MMU_CIRCpedia_217515, circAtlas: mmu-Zfp160_0001
Human Ortholog IDs:
Mature spliced Sequence:
GATTGACTTCTAAACATTAATGTTGCACAAGGAAGAACCTGGGAGAAGGAGAAGAAAGAGGAAATAAAAAAAAAAGCCAGGGATGGCTGTCACTCAGGTACAGGACAGTTGTCATTCAGTGATGTAGCCATAGAGTTCTCTCAAGAGGAATGGGAATGCCTAGACCATGCACAGAGAGCTTTGCACAGGGATGTCATGTTGGAGAATTACAGCAACCTGCTCTCCCTGGGAGTCACACTTCCAAATTTGAATGCTATGTCTAAGTTGGAACAATACAAAGGGCCCTGGACTGTGGAGAGTGAGGCAATAAGAACCAGAAAATCAAATGGAAAGGAATATATCAGAGCTATGAGTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTGGACTGTGGAGAGTGA
60.54
CTGAGTGACAGCCATCCCTG
60.11
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACTGTGGAGAGTGAGGCAA
59.32
CTGAGTGACAGCCATCCCTG
60.11
168
PCR template sequence:
TCTAAGTTGGAACAATACAAAGGGCCCTGGACTGTGGAGAGTGAGGCAATAAGAACCAGAAAATCAAATGGAAAGGAATATATCAGAGCTATGAGTGCAGGATTGACTTCTAAACATTAATGTTGCACAAGGAAGAACCTGGGAGAAGGAGAAGAAAGAGGAAATAAAAAAAAAAGCCAGGGATGGCTGTCACTCAGGTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-19a-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
GCTATGAGTGCAGGATTGACTTCTAA
siRNA sets
TTAGAAGTCAATCCTGCAC, TAGAAGTCAATCCTGCACT, TCAATCCTGCACTCATAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP160_358_ORF_1
0.6838
-0.022557187
0.059530695
183
ORF sequence:
ATGTTGGAGAATTACAGCAACCTGCTCTCCCTGGGAGTCACACTTCCAAATTTGAATGCTATGTCTAAGTTGGAACAATACAAAGGGCCCTGGACTGTGGAGAGTGAGGCAATAAGAACCAGAAAATCAAATGGAAAGGAATATATCAGAGCTATGAGTGCAGGATTGACTTCTAAACATTAA
Peptide sequence:
MLENYSNLLSLGVTLPNLNAMSKLEQYKGPWTVESEAIRTRKSNGKEYIRAMSAGLTSKH
IRESfinder result
IRESfinder score
0.373482249
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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