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mmu_circZfp148_567
Basic information of circular RNA
PanCircBase ID:
mmu_circZfp148_567
mm39_Chr. Position ID:
chr16|33241690|33255344|+
Gene name:
Zfp148
Transcript:
ENSMUST00000165418.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,5873,13305
Genomic length:
13654
Exon/intron sizes in nt:
82,136,349
Flanking exon/introns:
chr16:33201397-33241690|chr16:33255344-33277245
Splice length:
567
Other database IDs:
circBase: mmu_circ_0000684, CIRCpedia: MMU_CIRCpedia_16907, circAtlas: mmu-Zfp148_0015
circBase: hsa_circ_0001333, circAtlas: hsa-ZNF148_0013, CIRCpedia: HSA_CIRCpedia_43339
Human Ortholog IDs:
Mature spliced Sequence:
GATAGAAGAGAAGAATCAGTGGTTTGGAAATTTAGCCAAGTGATCTGGCGGGATGGGCTTATACCTGACACATTTTATAGAGGCATTGGATGGCACAAGTGACTGAGAGGATCCCGAGAGTTTCCGGGGCCTGTGAAAGGTAGAAACTCAACCATGATTTCTTTCTCAACTCAACAGCATTCCCTTCCTTGAAGTCTTCGTTTTTACTTCAGCCTCAGGCAGTTATACTTAAGCATGAACATTGACGACAAACTGGAAGGATTGTTTCTTAAATGTGGCGGCATAGACGAAATGCAGTCTTCCAGGGCAATGGTTGTAATGGGTGGAGTGTCTGGCCAGTCCGCCGTGTCTGGAGAACTGCAGGAGTCAGTACTTCAAGATCGAAGTTTGCCTCACCAGGAGATCCTTGCTGCAGACGAAGTGTTACAAGAGAGTGAAATGAGACAACAGGATATGATATCCCATGATGAACTCATGGTCCACGAGGAGACAGTGAAAAATGACGAAGAGCAGATGGACACCCACGAGCGGCTTCCCCAAGGACTGCAGTATGCACTCAATGTCCCT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCATGGTCCACGAGGAGA
59.96
CCGCCAGATCACTTGGCTAA
60.11
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CATGGTCCACGAGGAGACAG
59.83
CCGCCAGATCACTTGGCTAA
60.11
145
PCR template sequence:
TGAACTCATGGTCCACGAGGAGACAGTGAAAAATGACGAAGAGCAGATGGACACCCACGAGCGGCTTCCCCAAGGACTGCAGTATGCACTCAATGTCCCTGATAGAAGAGAAGAATCAGTGGTTTGGAAATTTAGCCAAGTGATCTGGCGGGATGGGCTTATACCTGACACATTTTATAGAGGCATTGGATGGCACAAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-7a-5p(1), mmu-miR-193b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTCAATGTCCCTGATAGAAGAGAAG
siRNA sets
TTCTATCAGGGACATTGAG, TCTATCAGGGACATTGAGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCZFP148_567_ORF_1
1.1713
0.204759653
0.707830196
435
ORF sequence:
ATGAACATTGACGACAAACTGGAAGGATTGTTTCTTAAATGTGGCGGCATAGACGAAATGCAGTCTTCCAGGGCAATGGTTGTAATGGGTGGAGTGTCTGGCCAGTCCGCCGTGTCTGGAGAACTGCAGGAGTCAGTACTTCAAGATCGAAGTTTGCCTCACCAGGAGATCCTTGCTGCAGACGAAGTGTTACAAGAGAGTGAAATGAGACAACAGGATATGATATCCCATGATGAACTCATGGTCCACGAGGAGACAGTGAAAAATGACGAAGAGCAGATGGACACCCACGAGCGGCTTCCCCAAGGACTGCAGTATGCACTCAATGTCCCTGATAGAAGAGAAGAATCAGTGGTTTGGAAATTTAGCCAAGTGATCTGGCGGGATGGGCTTATACCTGACACATTTTATAGAGGCATTGGATGGCACAAGTGA
Peptide sequence:
MNIDDKLEGLFLKCGGIDEMQSSRAMVVMGGVSGQSAVSGELQESVLQDRSLPHQEILAADEVLQESEMRQQDMISHDELMVHEETVKNDEEQMDTHERLPQGLQYALNVPDRREESVVWKFSQVIWRDGLIPDTFYRGIGWHK
IRESfinder result
IRESfinder score
0.61807374
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
RPF, cORF, m6A
mmu_circZfp148_001
Evidence
Peptide sequence:
MNIDDKLEGLFLKCGGIDEMQSSRAMVVMGGVSGQSAVSGELQESVLQDRSLPHQEILAADEVLQESEMRQQDMISHDELMVHEETVKNDEEQMDTHERLPQGLQYALNVPDRREESVVWKFSQVIWRDGLIPDTFYRGIGWHK*
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